March 20, 2024  |  RNA sequencing

SQANTI3: curation of long-read transcriptomes for accurate identification of known and novel isoforms

Authors: Francisco J. Pardo-Palacios, Angeles Arzalluz-Luque, Liudmyla Kondratova, Pedro Salguero, Jorge Mestre-Tomás, Rocío Amorín, Eva Estevan-Morió, Tianyuan Liu, Adalena Nanni, Lauren McIntyre, Elizabeth Tseng & Ana Conesa

SQANTI3 is a tool designed for the quality control, curation and annotation of long-read transcript models obtained with third-generation sequencing technologies. Leveraging its annotation framework, SQANTI3 calculates quality descriptors of transcript models, junctions and transcript ends. With this information, potential artifacts can be identified and replaced with reliable sequences. Furthermore, the integrated functional annotation feature enables subsequent functional iso-transcriptomics analyses.

Journal: Nature Methods
DOI: 10.1038/s41592-024-02229-2
Year: 2024

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