July 7, 2019  |  

Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians.

Authors: Grohme, Markus A and Vila-Farré, Miquel and Rink, Jochen C

High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.

Journal: Methods in molecular biology
DOI: 10.1007/978-1-4939-7802-1_7
Year: 2018

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