October 23, 2019  |  

Short DNA hairpins compromise recombinant adeno-associated virus genome homogeneity.

Authors: Xie, Jun and Mao, Qin and Tai, Phillip W L and He, Ran and Ai, Jianzhong and Su, Qin and Zhu, Ye and Ma, Hong and Li, Jia and Gong, Shoufang and Wang, Dan and Gao, Zhen and Li, Mengxin and Zhong, Li and Zhou, Heather and Gao, Guangping

Short hairpin (sh)RNAs delivered by recombinant adeno-associated viruses (rAAVs) are valuable tools to study gene function in vivo and a promising gene therapy platform. Our data show that incorporation of shRNA transgenes into rAAV constructs reduces vector yield and produces a population of truncated and defective genomes. We demonstrate that sequences with hairpins or hairpin-like structures drive the generation of truncated AAV genomes through a polymerase redirection mechanism during viral genome replication. Our findings reveal the importance of genomic secondary structure when optimizing viral vector designs. We also discovered that shDNAs could be adapted to act as surrogate mutant inverted terminal repeats (mTRs), sequences that were previously thought to be required for functional self-complementary AAV vectors. The use of shDNAs as artificial mTRs opens the door to engineering a new generation of AAV vectors with improved potency, genetic stability, and safety for both preclinical studies and human gene therapy. Published by Elsevier Inc.

Journal: Molecular therapy
DOI: 10.1016/j.ymthe.2017.03.028
Year: 2017

Read Publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.