Rapid sequencing of complete env genes from primary HIV-1 samples

Authors: Laird Smith, Melissa and Murrell, Ben and Eren, Kemal and Ignacio, Caroline and Landais, Elise and Weaver, Steven and Phung, Pham and Ludka, Colleen and Hepler, Lance and Caballero, Gemma and Pollner, Tristan and Guo, Yan and Richman, Douglas and , and Poignard, Pascal and Paxinos, Ellen E. and Kosakovsky Pond, Sergei L. and Smith, Davey M.

The ability to study rapidly evolving viral populations has been constrained by the read length of next-generation sequencing approaches and the sampling depth of single-genome amplification methods. Here, we develop and characterize a method using Pacific Biosciences Single Molecule, Real-Time (SMRT) sequencing technology to sequence multiple, intact full-length human immunodeficiency virus-1 env genes amplified from viral RNA populations circulating in blood, and provide computational tools for analyzing and visualizing these data.

Journal: Virus evolution
DOI: 10.1093/ve/vew018
Year: 2016

Read publication