July 19, 2019  |  

Polymerase specific error rates and profiles identified by single molecule sequencing.

Authors: Hestand, Matthew S and Houdt, Jeroen Van and Cristofoli, Francesca and Vermeesch, Joris R

DNA polymerases have an innate error rate which is polymerase and DNA context specific. Historically the mutational rate and profiles have been measured using a variety of methods, each with their own technical limitations. Here we used the unique properties of single molecule sequencing to evaluate the mutational rate and profiles of six DNA polymerases at the sequence level. In addition to accurately determining mutations in double strands, single molecule sequencing also captures direction specific transversions and transitions through the analysis of heteroduplexes. Not only did the error rates vary, but also the direction specific transitions differed among polymerases. Copyright © 2016 Elsevier B.V. All rights reserved.

Journal: Mutation research
DOI: 10.1016/j.mrfmmm.2016.01.003
Year: 2016

Read publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.