September 22, 2019  |  

Long-read, Single Molecule, Real-Time (SMRT) DNA Sequencing for metagenomic applications

Authors: Bowman, B. and Kim, M. and Cho, Y. and Korlach, J

In this chapter, we describe applications of single molecule, real-time (SMRT) DNA sequencing toward metagenomic research. The long sequence reads, combined with a lack of bias with respect to DNA sequence context or GC content, facilitate a more comprehensive analysis of the genomic constitution of microbial communities. Full-length 16S RNA gene sequencing at high (>99%) accuracy allows for species-level characterization of community members concomitant with the determination of community structure. The application of SMRT sequencing to whole-community shotgun microbial metagenomics has also been discussed.

DOI: 10.1016/B978-0-12-410472-3.00002-6
Year: 2015

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