July 7, 2019  |  

Library construction for high-throughput mobile element identification and genotyping.

Authors: Ha, Hongseok and Wang, Nan and Xing, Jinchuan

Mobile genetic elements are discrete DNA elements that can move around and copy themselves in a genome. As a ubiquitous component of the genome, mobile elements contribute to both genetic and epigenetic variation. Therefore, it is important to determine the genome-wide distribution of mobile elements. Here we present a targeted high-throughput sequencing protocol called Mobile Element Scanning (ME-Scan) for genome-wide mobile element detection. We will describe oligonucleotides design, sequencing library construction, and computational analysis for the ME-Scan protocol.

Journal: Methods in molecular biology
DOI: 10.1007/7651_2015_265
Year: 2015

Read publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.