April 21, 2020  |  

Identification of a Xist silencing domain by Tiling CRISPR.

Authors: Wang, Yang and Zhong, Yang and Zhou, Yingyao and Tanaseichuk, Olga and Li, Zhizhong and Zhao, Jing Crystal

Despite essential roles played by long noncoding RNAs (lncRNAs) in development and disease, methods to determine lncRNA cis-elements are lacking. Here, we developed a screening method named "Tiling CRISPR" to identify lncRNA functional domains. Using this approach, we identified Xist A-Repeats as the silencing domain, an observation in agreement with published work, suggesting Tiling CRISPR feasibility. Mechanistic analysis suggested a novel function for Xist A-repeats in promoting Xist transcription. Overall, our method allows mapping of lncRNA functional domains in an unbiased and potentially high-throughput manner to facilitate the understanding of lncRNA functions.

Journal: Scientific reports
DOI: 10.1038/s41598-018-36750-0
Year: 2019

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