G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.

Authors: Macaulay, Iain C and Haerty, Wilfried and Kumar, Parveen and Li, Yang I and Hu, Tim Xiaoming and Teng, Mabel J and Goolam, Mubeen and Saurat, Nathalie and Coupland, Paul and Shirley, Lesley M and Smith, Miriam and Van der Aa, Niels and Banerjee, Ruby and Ellis, Peter D and Quail, Michael A and Swerdlow, Harold P and Zernicka-Goetz, Magdalena and Livesey, Frederick J and Ponting, Chris P and Voet, Thierry

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

Journal: Nature methods
DOI: 10.1038/nmeth.3370
Year: 2015

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