September 22, 2019  |  

Genome-wide transcriptome profiling of the medicinal plant Zanthoxylum planispinum using a single-molecule direct RNA sequencing approach.

Authors: Kim, Jung-A and Roy, Neha Samir and Lee, Inn-Hye and Choi, Ah-Young and Choi, Beom-Soon and Yu, Yei-Soo and Park, Nam-Il and Park, Kyong-Cheul and Kim, Soonok and Yang, Hee-Sun and Choi, Ik-Young

High-throughput RNA sequencing has revolutionized transcriptome-based studies of candidate genes, key pathways and gene regulation in non-model organisms. We analyzed full-length cDNA sequences in Zanthoxylum planispinum (Z. planispinum), a medicinal herb in major parts of East Asia. The full-length mRNA derived from tissues of leaf, early fruit and maturing fruit stage were sequenced using PacBio RSII platform to identify isoform transcriptome. We obtained 51,402 unigenes, with average 1781?bp per gene in 82.473?Mb gene lengths. Among 51,402, 3963 unigenes showed variety of isoform. By selection of one representative gene among each of the various isoforms, we finalized 46,306 unique gene set for this herb. We identified 76 cytochrome P450 (CYP450) and related isoforms that are of the wide diversity in the molecular function and biological process. These transcriptome data of Z. planispinum will provide a good resource to study metabolic engineering for the production of valuable medicinal drugs and phytochemicals. Copyright © 2018. Published by Elsevier Inc.

Journal: Genomics
DOI: 10.1016/j.ygeno.2018.06.004
Year: 2018

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