July 7, 2019  |  

Genome editing in human pluripotent stem cells: approaches, pitfalls, and solutions.

Authors: Hendriks, William T and Warren, Curtis R and Cowan, Chad A

Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design. Copyright © 2016 Elsevier Inc. All rights reserved.

Journal: Cell stem cell
DOI: 10.1016/j.stem.2015.12.002
Year: 2016

Read Publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.