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Authors: Lin, Runmao and Qin, Feifei and Shen, Baoming and Shi, Qianqian and Liu, Chichuan and Zhang, Xi and Jiao, Yang and Lu, Jun and Gao, Yaoyao and Suarez-Fernandez, Marta and Lopez-Moya, Federico and Lopez-Llorca, Luis Vicente and Wang, Gang and Mao, Zhenchuan and Ling, Jian and Yang, Yuhong and Cheng, Xinyue and Xie, Bingyan

Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (~44?Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (~41?Mb) isolated from cereal cyst nematode. We focus on secretomes of the fungus, which play important roles in pathogenicity and fungus-host/environment interactions, and identified 1,750 secreted proteins, with a high proportion of carboxypeptidases, subtilisins, and chitinases. We analyzed the phylogenies of these genes and predicted new pathogenic molecules. By comparative transcriptome analysis, we found that secreted proteins involved in responses to nutrient stress are mainly comprised of proteases and glycoside hydrolases. Moreover, 32 secreted proteins undergoing positive selection and 71 duplicated gene pairs encoding secreted proteins are identified. Two duplicated pairs encoding secreted glycosyl hydrolases (GH30), which may be related to fungal endophytic process and lost in many insect-pathogenic fungi but exist in nematophagous fungi, are putatively acquired from bacteria by horizontal gene transfer. The results help understanding genetic origins and evolution of parasitism-related genes.

Journal: Scientific Reports
DOI: 10.1038/s41598-018-19169-5
Year: 2018

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