July 19, 2019  |  

Genome analysis of the fruiting body forming myxobacterium Chondromyces crocatus reveals high potential for natural product biosynthesis.

Authors: Zaburannyi, Nestor and Bunk, Boyke and Maier, Josef and Overmann, Jörg and Müller, Rolf

Here we report the first complete genome sequence of the type strain of the myxobacterial genus Chondromyces - Chondromyces crocatus Cm c5. It presents one of the largest prokaryotic genomes featuring a single circular chromosome and no plasmids. Analysis revealed an enlarged set of tRNA genes, along with reduced pressure on preferred codon usage compared to other bacterial genomes. The large coding capacity and the plethora of encoded secondary metabolite biosynthetic gene clusters is in line with the capability of Cm c5 to produce an arsenal of anti-bacterial, anti-fungal and cytotoxic compounds. Known pathways of the ajudazol, chondramide, chondrochloren, crocacin, crocapeptin and thuggacin compound families are complemented by many more natural compound biosynthetic gene clusters in the chromosome. Whole-genome comparison of the fruiting-body forming type-strain (Cm c5 = DSM 14714) to an accustomed laboratory strain which has lost this ability (Cm c5 fr-) revealed genetic changes in three loci. In addition to the low synteny found with the closest sequenced representative of the same family, Sorangium cellulosum, extensive genetic information duplication, and broad application of eukaryotic-type signal transduction systems are hallmarks of this 11.3 Mbp prokaryotic genome. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Journal: Applied and environmental microbiology
DOI: 10.1128/AEM.03011-15
Year: 2016

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