Conjugal Transfer, Whole-Genome Sequencing, and Plasmid Analysis of Four mcr-1-Bearing Isolates from U.S. Patients.

Authors: Zhu, Wenming and Lawsin, Adrian and Lindsey, Rebecca L and Batra, Dhwani and Knipe, Kristen and Yoo, Brian B and Perry, K Allison and Rowe, Lori A and Lonsway, David and Walters, Maroya S and Rasheed, J Kamile and Halpin, Alison Laufer

Four Enterobacteriaceae clinical isolates bearing mcr-1 gene-harboring plasmids were characterized. All isolates demonstrated the ability to transfer colistin resistance to Escherichia coli; plasmids were stable in conjugants after multiple passages on nonselective media. mcr-1 was located on an IncX4 (n?=?3) or IncN (n?=?1) plasmid. The IncN plasmid harbored 13 additional antimicrobial resistance genes. Results indicate that the mcr-1-bearing plasmids in this study were highly transferable in vitro and stable in the recipients.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

Journal: Antimicrobial agents and chemotherapy
DOI: 10.1128/AAC.02417-18
Year: 2019

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