Complete genome sequences of eight Helicobacter pylori strains with different virulence factor genotypes and methylation profiles, isolated from patients with diverse gastrointestinal diseases on Okinawa Island, Japan, determined using PacBio Single-Molecule Real-Time Technology.

Authors: Satou, Kazuhito and Shiroma, Akino and Teruya, Kuniko and Shimoji, Makiko and Nakano, Kazuma and Juan, Ayaka and Tamotsu, Hinako and Terabayashi, Yasunobu and Aoyama, Misako and Teruya, Morimi and Suzuki, Rumiko and Matsuda, Miyuki and Sekine, Akihiro and Kinjo, Nagisa and Kinjo, Fukunori and Yamaoka, Yoshio and Hirano, Takashi

We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs.

Journal: Genome announcements
DOI: 10.1128/genomeA.00286-14
Year: 2014

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