July 7, 2019  |  

Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T).

Authors: Bosma, Elleke F and Koehorst, Jasper J and van Hijum, Sacha A F T and Renckens, Bernadet and Vriesendorp, Bastienne and van de Weijer, Antonius H P and Schaap, Peter J and de Vos, Willem M and van der Oost, John and van Kranenburg, Richard

Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of the B. smithii type strain DSM 4216(T), which consists of a 3,368,778 bp chromosome (GenBank accession number CP012024.1) and a 12,514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features of B. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.

Journal: Standards in genomic sciences
DOI: 10.1186/s40793-016-0172-8
Year: 2016

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