April 21, 2020  |  

Combinations of Spok genes create multiple meiotic drivers in Podospora.

Authors: Vogan, Aaron A and Ament-Velásquez, S Lorena and Granger-Farbos, Alexandra and Svedberg, Jesper and Bastiaans, Eric and Debets, Alfons Jm and Coustou, Virginie and Yvanne, Hélène and Clavé, Corinne and Saupe, Sven J and Johannesson, Hanna

Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the Spok gene family underlies the Psks. The combination of Spok genes at different chromosomal locations defines the spore killer types and creates a killing hierarchy within a population. We identify two novel Spok homologs located within a large (74-167 kbp) region (the Spok block) that resides in different chromosomal locations in different strains. We confirm that the SPOK protein performs both killing and resistance functions and show that these activities are dependent on distinct domains, a predicted nuclease and kinase domain. Genomic and phylogenetic analyses across ascomycetes suggest that the Spok genes disperse through cross-species transfer, and evolve by duplication and diversification within lineages. © 2019, Vogan et al.

Journal: eLife
DOI: 10.7554/eLife.46454
Year: 2019

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