July 7, 2019  |  

Assembly of an early-matured japonica (Geng) rice genome, Suijing18, based on PacBio and Illumina sequencing.

Authors: Nie, Shou-Jun and Liu, Yu-Qiang and Wang, Chun-Chao and Gao, Shi-Wei and Xu, Tian-Tian and Liu, Qing and Chang, Hui-Lin and Chen, Yu-Bao and Yan, Peng-Cheng and Peng, Wei and Zheng, Tian-Qing and Xu, Jian-Long and Li, Zhi-Kang

The early-matured japonica (Geng) rice variety, Suijing18 (SJ18), carries multiple elite traits including durable blast resistance, good grain quality, and high yield. Using PacBio SMRT technology, we produced over 25?Gb of long-read sequencing raw data from SJ18 with a coverage of 62×. Using Illumina paired-end whole-genome shotgun sequencing technology, we generated 59?Gb of short-read sequencing data from SJ18 (23.6?Gb from a 200?bp library with a coverage of 59× and 35.4?Gb from an 800?bp library with a coverage of 88×). With these data, we assembled a single SJ18 genome and then generated a set of annotation data. These data sets can be used to test new programs for variation deep mining, and will provide new insights into the genome structure, function, and evolution of SJ18, and will provide essential support for biological research in general.

Journal: Scientific data
DOI: 10.1038/sdata.2017.195
Year: 2017

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