July 7, 2019  |  

A phylogenetic and phenotypic analysis of Salmonella enterica serovar Weltevreden, an emerging agent of diarrheal disease in tropical regions.

Authors: Makendi, Carine and Page, Andrew J and Wren, Brendan W and Le Thi Phuong, Tu and Clare, Simon and Hale, Christine and Goulding, David and Klemm, Elizabeth J and Pickard, Derek and Okoro, Chinyere and Hunt, Martin and Thompson, Corinne N and Phu Huong Lan, Nguyen and Tran Do Hoang, Nhu and Thwaites, Guy E and Le Hello, Simon and Brisabois, Anne and Weill, François-Xavier and Baker, Stephen and Dougan, Gordon

Salmonella enterica serovar Weltevreden (S. Weltevreden) is an emerging cause of diarrheal and invasive disease in humans residing in tropical regions. Despite the regional and international emergence of this Salmonella serovar, relatively little is known about its genetic diversity, genomics or virulence potential in model systems. Here we used whole genome sequencing and bioinformatics analyses to define the phylogenetic structure of a diverse global selection of S. Weltevreden. Phylogenetic analysis of more than 100 isolates demonstrated that the population of S. Weltevreden can be segregated into two main phylogenetic clusters, one associated predominantly with continental Southeast Asia and the other more internationally dispersed. Subcluster analysis suggested the local evolution of S. Weltevreden within specific geographical regions. Four of the isolates were sequenced using long read sequencing to produce high quality reference genomes. Phenotypic analysis in Hep-2 cells and in a murine infection model indicated that S. Weltevreden were significantly attenuated in these models compared to the classical S. Typhimurium reference strain SL1344. Our work outlines novel insights into this important emerging pathogen and provides a baseline understanding for future research studies.

Journal: PLoS neglected tropical diseases
DOI: 10.1371/journal.pntd.0004446
Year: 2016

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