A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana.

Authors: Nowell, Reuben W and Elsworth, Ben and Oostra, Vicencio and Zwaan, Bas J and Wheat, Christopher W and Saastamoinen, Marjo and Saccheri, Ilik J and Van't Hof, Arjen E and Wasik, Bethany R and Connahs, Heidi and Aslam, Muhammad L and Kumar, Sujai and Challis, Richard J and Monteiro, Antónia and Brakefield, Paul M and Blaxter, Mark

The mycalesine butterfly Bicyclus anynana , the 'Squinting bush brown', is a model organism in the study of lepidopteran ecology, development and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species.Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology. 128 Gb raw Illumina data were filtered to 124 Gb and assembled to a final size of 475 Mb (~260X assembly coverage). Contigs were scaffolded using mate-pair, transcriptome and PacBio data into 10,800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements, and encodes a total of 22,642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes.We report a high-quality draft genome sequence for Bicyclus anynana . The genome assembly and annotated gene models are available at LepBase ( http://ensembl.lepbase.org/index.html ).

Journal: GigaScience
DOI: 10.1093/gigascience/gix035
Year: 2017

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