July 7, 2019  |  

A Clostridioides difficile bacteriophage genome encodes functional binary toxin-associated genes.

Authors: Riedel, Thomas and Wittmann, Johannes and Bunk, Boyke and Schober, Isabel and Spröer, Cathrin and Gronow, Sabine and Overmann, Jörg

Pathogenic clostridia typically produce toxins as virulence factors which cause severe diseases in both humans and animals. Whereas many clostridia like e.g., Clostridium perfringens, Clostridium botulinum or Clostridium tetani were shown to contain toxin-encoding plasmids, only toxin genes located on the chromosome were detected in Clostridioides difficile so far. In this study, we determined, annotated, and analyzed the complete genome of the bacteriophage phiSemix9P1 using single-molecule real-time sequencing technology (SMRT). To our knowledge, this represents the first C. difficile-associated bacteriophage genome that carries a complete functional binary toxin locus in its genome. Copyright © 2017 Elsevier B.V. All rights reserved.

Journal: Journal of biotechnology
DOI: 10.1016/j.jbiotec.2017.02.017
Year: 2017

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