SMRT Cells, Sequencing Reagent Kits, and Accessories

The SMRT Cell 8M Tray contains 4 SMRT Cells to be used with the Sequel II and Sequel IIe Systems. Each is a nanofabricated consumable patterned with approximately 8,000,000 wells called zero-mode waveguides (ZMWs). One SMRT Cell is utilized for each sequencing reaction. One SMRT Cell is utilized in each sequencing collection, and experiments can be run on a single collection or in batch mode, giving your projects a greater level of flexibility.

Supports 4 SMRT Sequencing collections.

Notes: Sequel II and Sequel IIe Systems compatible only.

• SDS: SMRT Cell 8M Tray

The Sequel II Sequencing 2.0 Bundle (4 rxn - 5 pack) contains reagent and disposable supplies for 20 reactions of SMRT Sequencing. This bundle includes: five Sequel II Sequencing Kit 2.0 (4 rxn) (PN: 101-820-200), one Sequel SMRT Cell Oil pack (PN:100-621-300, and one Tube Septa pack (PN: 100-667-700). The Sequel II Sequencing Kit 2.0 is to be used with a Sequel II System SMRT Cell 8M. Sequel Sequencing Kit 2.0 is compatible with templates bound to Sequel II DNA Polymerase 2.0, Sequel II DNA Polymerase 2.1 and Sequel II DNA Polymerase 2.2.

Supports 20 SMRT Sequencing collections.

Notes: Sequel II and Sequel IIe Systems compatible only. Kit components also sold separately.

• Product insert: Sequel II Sequencing Kit 2.0 (4rxn)
• Product insert: SMRT Cell Oil
• SDS: Sequel II Sequencing Kit 2.0 (4rxn)
• SDS: SMRT Cell Oil

The Sequel II Sequencing Kit 2.0 (4 rxn) contains a sequencing reagent plate with Sequel II Sequencing Chemistry 2.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme, for SMRT Sequencing on the Sequel II and Sequel IIe Systems. The Sequel II Sequencing Kit 2.0 is to be used with a Sequel II System SMRT Cell 8M. Sequel Sequencing Kit 2.0 is compatible with templates bound to Sequel II DNA Polymerase 2.0, Sequel II DNA Polymerase 2.1 and Sequel II DNA Polymerase 2.2.

Supports 4 SMRT Sequencing collections.

Notes: Sequel II and Sequel IIe Systems compatible only.

• Product insert: Sequel II Sequencing Kit 2.0 (4rxn)
• SDS: Sequel II Sequencing Kit 2.0 (4rxn)

The Sequel SMRT Cell 1M v3 is a set of 4 nanofabricated consumables each patterned with approximately 1,000,000 wells called zero-mode waveguides (ZMWs). The SMRT Cell 1M v3 can acquire sequencing data up to 10 hours of collection time. SMRT Cell 1M v3 is compatible with Sequel Binding and Internal Control Kit v3.0 (PN: 101-626-600) and Sequel Sequencing Kit 3.0 4 and 8 rxn (PN:101-597-900/-800). One SMRT Cell is utilized in each sequencing collection, and up to 12 SMRT Cells can be run at a time giving you flexibility in how to set up your experiments.

Supports 4 SMRT Sequencing collections.

Note: Sequel System compatible only.

• SDS: SMRT-Cell-1M-4tray

The Sequel SMRT Cell 1M v3 LR Tray is a set of 4 nanofabricated consumables each patterned with approximately 1,000,000 wells called zero-mode waveguides (ZMWs). Compared to the SMRT Cell 1M v3, SMRT Cell 1M v3 LR can acquire sequencing data for a longer collection time, up to 20 hours. SMRT Cell 1M v3 LR is compatible with Sequel Binding and Internal Control Kit v3.0 (PN: 101-626-600) and Sequel Sequencing Kit 3.0 4 and 8 rxn (PN:101-597-900/-800). One SMRT Cell is utilized in each sequencing reaction, and up to 12 SMRT Cells can be run at a time giving you flexibility in how to set up your experiments.

Supports 4 SMRT Sequencing collections.

Note: Sequel System compatible only.

• SDS: SMRT-Cell-1M-4tray

The Sequel Sequencing 3.0 Bundle (5 pack - 4 reactions) contains reagent and disposable supplies for 20 reactions of SMRT Sequencing. It contains five Sequel Sequencing Kits 3.0 - 4 reaction plate (PN: 101-597-800), one Sequel SMRT Cell Oil pack (PN:100-621-300, and one Tube Septa pack (PN: 100-667-700). Use with Sequel SMRT Cell 1M v3 (PN: 101-531-000) and Sequel SMRT Cell 1M v3 LR (PN:101-531-001) and templates prepared with Sequel Binding and Control Kit 3.0 (PN:101-626-600) to achieve up to 20 Gb for large-insert libraries and more for other applications.

Supports 20 SMRT Sequencing reactions.

Note: Sequel System compatible only. Kit components also sold separately.

• Product insert: Sequel Sequencing Kit 3.0 (4 rxn)
• Product insert: SMRT Cell Oil
• SDS: Sequel Sequencing Kit 3.0 (4 rxn)
• SDS: SMRT Cell Oil

The Sequel Sequencing 3.0 Bundle (5 pack - 8 reactions) contains reagent and disposable supplies for 40 reactions of SMRT Sequencing. It contains five Sequel Sequencing Kits 3.0 - 8 reactions (PN:101-597-900), one Sequel SMRT Cell Oil pack (PN:100-621-300, and one Tube Septa pack (PN: 100-667-700). Use with Sequel SMRT Cell 1M v3 (PN: 101-531-000) and Sequel SMRT Cell 1M v3 LR (PN:101-531-001) and templates prepared with Sequel Binding and Control Kit 3.0 (PN:101-626-600) to achieve up to 20 Gb for large-insert libraries and more for other applications. To be discontinued on December 31, 2019.

Supports 40 SMRT Sequencing reactions.

Note: Sequel System compatible only. Kit components also sold separately.

• Product insert: Sequel Sequencing Kit 3.0 (8 rxn)
• Product insert: SMRT Cell Oil
• SDS: Sequel Sequencing Kit 3.0 (8 rxn)
• SDS: SMRT Cell Oil

The Sequel Sequencing Kit 3.0 (4 reactions) contains a sequencing reagent plate with Sequel Sequencing chemistry 3.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme, for SMRT Sequencing on the Sequel System. Use with Sequel SMRT Cell 1M v3 (PN: 101-531-000) and Sequel SMRT Cell 1M v3 LR (PN:101-531-001) and templates prepared with Sequel Binding and Control Kit 3.0 (PN:101-626-600) to achieve up to 20 Gb for large-insert libraries and more for other applications.

Supports 4 SMRT Sequencing reactions.

Note: Sequel System compatible only.

• Product insert: Sequel Sequencing Kit 3.0 (4 rxn)
• SDS: Sequel Sequencing Kit 3.0 (4 rxn)

The Sequel Sequencing Kit 3.0 (8 reactions) contains a sequencing reagent plate with Sequel Sequencing chemistry 3.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme, for SMRT Sequencing on the Sequel System. Use with Sequel SMRT Cell 1M v3 (PN: 101-531-000) and Sequel SMRT Cell 1M v3 LR (PN:101-531-001) and templates prepared with Sequel Binding and Control Kit 3.0 (PN:101-626-600) to achieve up to 20 Gb for large-insert libraries and more for other ap plications. To be discontinued on December 31, 2019.

Supports 8 SMRT Sequencing reactions.

Note: Sequel System compatible only.

• Product insert: Sequel Sequencing Kit 3.0 (8 rxn)
• SDS: Sequel Sequencing Kit 3.0 (8 rxn)

The Sequel SMRT Cell Oil contains 5 reagent tubes for environmental control and evaporation mitigation from SMRT Cells during SMRT Sequencing on Sequel Systems.

Reagent quantities support 5 sequencing plate runs.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

• Product insert: SMRT Cell Oil
• SDS: SMRT Cell Oil

Sequel Pipet Tips v2 have a tip rack that secures the pipet tips in the tip box. It contains filtered tips approved for robotic liquid handling on Sequel Systems.

Each case contains 40 racks of 96 tips each.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

Case of 96-well PCR plates approved for sample placement on Sequel Systems.

Each case contains 50 plates.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

Case of 96-well deep-well plates contains approved for reagent and sample mixing on Sequel Systems.

Each case contains 120 plates.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

Case of foil seals approved for evaporation and environmental control of the Sequel Sample Plate. The foil seals are heat-treated to cover the Sequel Sample Plate.

Each case contains 100 seals.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

Tube septa approved for evaporation control of Oxygen Scavenging (OS) Enzyme and SMRT Cell Oil tubes on the Sequel Systems.

Each bag contains 10 tube septa.

Note: Sequel, Sequel II and Sequel IIe Systems compatible.