August 17, 2026 | Procedure & checklist

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

August 17, 2026

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.

.navbar .megamenu{padding:0}@media all and ( min-width: 992px ){.navbar .has-megamenu{position:static !important}.navbar .megamenu{left:0;right:0;width:100%;margin-top:0}}@media (max-width: 991px){.navbar.fixed-top .navbar-collapse,.navbar.sticky-top .navbar-collapse{overflow-y:auto;max-height:90vh;margin-top:0px}}@media all and (min-width: 992px){.navbar .nav-item .dropdown-menu{display:none}.navbar .nav-item:hover .nav-link{}.navbar .nav-item:hover .dropdown-menu{display:block}.navbar .nav-item .dropdown-menu{margin-top:0}}img:is([sizes=auto i],[sizes^="auto," i]){contain-intrinsic-size:3000px 1500px}img.wp-smiley,img.emoji{display:inline !important;border:none !important;box-shadow:none !important;height:1em !important;width:1em !important;margin:0 .07em !important;vertical-align:-.1em !important;background:none !important;padding:0 !important}:root{--wp-block-synced-color:#7a00df;--wp-block-synced-color--rgb:122,0,223;--wp-bound-block-color:var(--wp-block-synced-color);--wp-editor-canvas-background:#ddd;--wp-admin-theme-color:#007cba;--wp-admin-theme-color--rgb:0,124,186;--wp-admin-theme-color-darker-10:#006ba1;--wp-admin-theme-color-darker-10--rgb:0,107,160.5;--wp-admin-theme-color-darker-20:#005a87;--wp-admin-theme-color-darker-20--rgb:0,90,135;--wp-admin-border-width-focus:2px}@media (min-resolution:192dpi){:root{--wp-admin-border-width-focus:1.5px}}.wp-element-button{cursor:pointer}:root .has-very-light-gray-background-color{background-color:#eee}:root .has-very-dark-gray-background-color{background-color:#313131}:root .has-very-light-gray-color{color:#eee}:root .has-very-dark-gray-color{color:#313131}:root .has-vivid-green-cyan-to-vivid-cyan-blue-gradient-background{background:linear-gradient(135deg,#00d084,#0693e3)}:root .has-purple-crush-gradient-background{background:linear-gradient(135deg,#34e2e4,#4721fb 50%,#ab1dfe)}:root .has-hazy-dawn-gradient-background{background:linear-gradient(135deg,#faaca8,#dad0ec)}:root .has-subdued-olive-gradient-background{background:linear-gradient(135deg,#fafae1,#67a671)}:root .has-atomic-cream-gradient-background{background:linear-gradient(135deg,#fdd79a,#004a59)}:root .has-nightshade-gradient-background{background:linear-gradient(135deg,#330968,#31cdcf)}:root .has-midnight-gradient-background{background:linear-gradient(135deg,#020381,#2874fc)}:root{--wp--preset--font-size--normal:16px;--wp--preset--font-size--huge:42px}.has-regular-font-size{font-size:1em}.has-larger-font-size{font-size:2.625em}.has-normal-font-size{font-size:var(--wp--preset--font-size--normal)}.has-huge-font-size{font-size:var(--wp--preset--font-size--huge)}:root .has-text-align-center{text-align:center}:root .has-text-align-left{text-align:left}:root .has-text-align-right{text-align:right}.has-fit-text{white-space:nowrap !important}#end-resizable-editor-section{display:none}.aligncenter{clear:both}.items-justified-left{justify-content:flex-start}.items-justified-center{justify-content:center}.items-justified-right{justify-content:flex-end}.items-justified-space-between{justify-content:space-between}.screen-reader-text{word-wrap:normal !important;border:0;clip-path:inset(50%);height:1px;margin:-1px;overflow:hidden;padding:0;position:absolute;width:1px;word-break:normal !important}.screen-reader-text:focus{background-color:#ddd;clip-path:none;color:#444;display:block;font-size:1em;height:auto;left:5px;line-height:normal;padding:15px 23px 14px;text-decoration:none;top:5px;width:auto;z-index:100000}html :where(.has-border-color){border-style:solid}html :where([style^=border-color],[style*=";border-color"],[style*="; border-color"]){border-style:solid}html :where([style^=border-top-color],[style*=";border-top-color"],[style*="; border-top-color"]){border-top-style:solid}html :where([style^=border-right-color],[style*=";border-right-color"],[style*="; border-right-color"]){border-right-style:solid}html :where([style^=border-bottom-color],[style*=";border-bottom-color"],[style*="; border-bottom-color"]){border-bottom-style:solid}html :where([style^=border-left-color],[style*=";border-left-color"],[style*="; border-left-color"]){border-left-style:solid}html :where([style^=border-width],[style*=";border-width"],[style*="; border-width"]){border-style:solid}html :where([style^=border-top-width],[style*=";border-top-width"],[style*="; border-top-width"]){border-top-style:solid}html :where([style^=border-right-width],[style*=";border-right-width"],[style*="; border-right-width"]){border-right-style:solid}html :where([style^=border-bottom-width],[style*=";border-bottom-width"],[style*="; border-bottom-width"]){border-bottom-style:solid}html :where([style^=border-left-width],[style*=";border-left-width"],[style*="; border-left-width"]){border-left-style:solid}html :where(img[class*=wp-image-]){height:auto;max-width:100%}:where(figure){margin:0 0 1em}html :where(.is-position-sticky){--wp-admin--admin-bar--position-offset:var(--wp-admin--admin-bar--height,0px)}@media screen and (max-width:600px){html :where(.is-position-sticky){--wp-admin--admin-bar--position-offset:0px}}.wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none}.svg-inline--fa{display:inline-block;height:1em;overflow:visible;vertical-align:-.125em}.wp-block-font-awesome-icon svg::before,.wp-rich-text-font-awesome-icon svg::before{content:unset}:root{--wp--preset--aspect-ratio--square:1;--wp--preset--aspect-ratio--4-3:4/3;--wp--preset--aspect-ratio--3-4:3/4;--wp--preset--aspect-ratio--3-2:3/2;--wp--preset--aspect-ratio--2-3:2/3;--wp--preset--aspect-ratio--16-9:16/9;--wp--preset--aspect-ratio--9-16:9/16;--wp--preset--color--black:#000;--wp--preset--color--cyan-bluish-gray:#abb8c3;--wp--preset--color--white:#fff;--wp--preset--color--pale-pink:#f78da7;--wp--preset--color--vivid-red:#cf2e2e;--wp--preset--color--luminous-vivid-orange:#ff6900;--wp--preset--color--luminous-vivid-amber:#fcb900;--wp--preset--color--light-green-cyan:#7bdcb5;--wp--preset--color--vivid-green-cyan:#00d084;--wp--preset--color--pale-cyan-blue:#8ed1fc;--wp--preset--color--vivid-cyan-blue:#0693e3;--wp--preset--color--vivid-purple:#9b51e0;--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple:linear-gradient(135deg,#0693e3 0%,#9b51e0 100%);--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan:linear-gradient(135deg,#7adcb4 0%,#00d082 100%);--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange:linear-gradient(135deg,#fcb900 0%,#ff6900 100%);--wp--preset--gradient--luminous-vivid-orange-to-vivid-red:linear-gradient(135deg,#ff6900 0%,#cf2e2e 100%);--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray:linear-gradient(135deg,#eee 0%,#a9b8c3 100%);--wp--preset--gradient--cool-to-warm-spectrum:linear-gradient(135deg,#4aeadc 0%,#9778d1 20%,#cf2aba 40%,#ee2c82 60%,#fb6962 80%,#fef84c 100%);--wp--preset--gradient--blush-light-purple:linear-gradient(135deg,#ffceec 0%,#9896f0 100%);--wp--preset--gradient--blush-bordeaux:linear-gradient(135deg,#fecda5 0%,#fe2d2d 50%,#6b003e 100%);--wp--preset--gradient--luminous-dusk:linear-gradient(135deg,#ffcb70 0%,#c751c0 50%,#4158d0 100%);--wp--preset--gradient--pale-ocean:linear-gradient(135deg,#fff5cb 0%,#b6e3d4 50%,#33a7b5 100%);--wp--preset--gradient--electric-grass:linear-gradient(135deg,#caf880 0%,#71ce7e 100%);--wp--preset--gradient--midnight:linear-gradient(135deg,#020381 0%,#2874fc 100%);--wp--preset--font-size--small:13px;--wp--preset--font-size--medium:20px;--wp--preset--font-size--large:36px;--wp--preset--font-size--x-large:42px;--wp--preset--spacing--20:.44rem;--wp--preset--spacing--30:.67rem;--wp--preset--spacing--40:1rem;--wp--preset--spacing--50:1.5rem;--wp--preset--spacing--60:2.25rem;--wp--preset--spacing--70:3.38rem;--wp--preset--spacing--80:5.06rem;--wp--preset--shadow--natural:6px 6px 9px rgba(0,0,0,.2);--wp--preset--shadow--deep:12px 12px 50px rgba(0,0,0,.4);--wp--preset--shadow--sharp:6px 6px 0px rgba(0,0,0,.2);--wp--preset--shadow--outlined:6px 6px 0px -3px #fff,6px 6px #000;--wp--preset--shadow--crisp:6px 6px 0px #000}.wp-block-button{--wp--preset--dimension--25:25%;--wp--preset--dimension--50:50%;--wp--preset--dimension--75:75%;--wp--preset--dimension--100:100%}:where(body){margin:0}:where(.is-layout-flex){gap:.5em}:where(.is-layout-grid){gap:.5em}body .is-layout-flex{display:flex}.is-layout-flex{flex-wrap:wrap;align-items:center}.is-layout-flex>:is(*, div){margin:0}body .is-layout-grid{display:grid}.is-layout-grid>:is(*, div){margin:0}body{padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px}:root :where(.wp-element-button, .wp-block-button__link){background-color:#32373c;border-width:0;color:#fff;font-family:inherit;font-size:inherit;font-style:inherit;font-weight:inherit;letter-spacing:inherit;line-height:inherit;padding-top:calc(.667em + 2px);padding-right:calc(1.333em + 2px);padding-bottom:calc(.667em + 2px);padding-left:calc(1.333em + 2px);text-decoration:none;text-transform:inherit}.has-black-color{color:var(--wp--preset--color--black) !important}.has-cyan-bluish-gray-color{color:var(--wp--preset--color--cyan-bluish-gray) !important}.has-white-color{color:var(--wp--preset--color--white) !important}.has-pale-pink-color{color:var(--wp--preset--color--pale-pink) !important}.has-vivid-red-color{color:var(--wp--preset--color--vivid-red) !important}.has-luminous-vivid-orange-color{color:var(--wp--preset--color--luminous-vivid-orange) !important}.has-luminous-vivid-amber-color{color:var(--wp--preset--color--luminous-vivid-amber) !important}.has-light-green-cyan-color{color:var(--wp--preset--color--light-green-cyan) !important}.has-vivid-green-cyan-color{color:var(--wp--preset--color--vivid-green-cyan) !important}.has-pale-cyan-blue-color{color:var(--wp--preset--color--pale-cyan-blue) !important}.has-vivid-cyan-blue-color{color:var(--wp--preset--color--vivid-cyan-blue) !important}.has-vivid-purple-color{color:var(--wp--preset--color--vivid-purple) !important}.has-black-background-color{background-color:var(--wp--preset--color--black) !important}.has-cyan-bluish-gray-background-color{background-color:var(--wp--preset--color--cyan-bluish-gray) !important}.has-white-background-color{background-color:var(--wp--preset--color--white) !important}.has-pale-pink-background-color{background-color:var(--wp--preset--color--pale-pink) !important}.has-vivid-red-background-color{background-color:var(--wp--preset--color--vivid-red) !important}.has-luminous-vivid-orange-background-color{background-color:var(--wp--preset--color--luminous-vivid-orange) !important}.has-luminous-vivid-amber-background-color{background-color:var(--wp--preset--color--luminous-vivid-amber) !important}.has-light-green-cyan-background-color{background-color:var(--wp--preset--color--light-green-cyan) !important}.has-vivid-green-cyan-background-color{background-color:var(--wp--preset--color--vivid-green-cyan) !important}.has-pale-cyan-blue-background-color{background-color:var(--wp--preset--color--pale-cyan-blue) !important}.has-vivid-cyan-blue-background-color{background-color:var(--wp--preset--color--vivid-cyan-blue) !important}.has-vivid-purple-background-color{background-color:var(--wp--preset--color--vivid-purple) !important}.has-black-border-color{border-color:var(--wp--preset--color--black) !important}.has-cyan-bluish-gray-border-color{border-color:var(--wp--preset--color--cyan-bluish-gray) !important}.has-white-border-color{border-color:var(--wp--preset--color--white) !important}.has-pale-pink-border-color{border-color:var(--wp--preset--color--pale-pink) !important}.has-vivid-red-border-color{border-color:var(--wp--preset--color--vivid-red) !important}.has-luminous-vivid-orange-border-color{border-color:var(--wp--preset--color--luminous-vivid-orange) !important}.has-luminous-vivid-amber-border-color{border-color:var(--wp--preset--color--luminous-vivid-amber) !important}.has-light-green-cyan-border-color{border-color:var(--wp--preset--color--light-green-cyan) !important}.has-vivid-green-cyan-border-color{border-color:var(--wp--preset--color--vivid-green-cyan) !important}.has-pale-cyan-blue-border-color{border-color:var(--wp--preset--color--pale-cyan-blue) !important}.has-vivid-cyan-blue-border-color{border-color:var(--wp--preset--color--vivid-cyan-blue) !important}.has-vivid-purple-border-color{border-color:var(--wp--preset--color--vivid-purple) !important}.has-vivid-cyan-blue-to-vivid-purple-gradient-background{background:var(--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple) !important}.has-light-green-cyan-to-vivid-green-cyan-gradient-background{background:var(--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan) !important}.has-luminous-vivid-amber-to-luminous-vivid-orange-gradient-background{background:var(--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange) !important}.has-luminous-vivid-orange-to-vivid-red-gradient-background{background:var(--wp--preset--gradient--luminous-vivid-orange-to-vivid-red) !important}.has-very-light-gray-to-cyan-bluish-gray-gradient-background{background:var(--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray) !important}.has-cool-to-warm-spectrum-gradient-background{background:var(--wp--preset--gradient--cool-to-warm-spectrum) !important}.has-blush-light-purple-gradient-background{background:var(--wp--preset--gradient--blush-light-purple) !important}.has-blush-bordeaux-gradient-background{background:var(--wp--preset--gradient--blush-bordeaux) !important}.has-luminous-dusk-gradient-background{background:var(--wp--preset--gradient--luminous-dusk) !important}.has-pale-ocean-gradient-background{background:var(--wp--preset--gradient--pale-ocean) !important}.has-electric-grass-gradient-background{background:var(--wp--preset--gradient--electric-grass) !important}.has-midnight-gradient-background{background:var(--wp--preset--gradient--midnight) !important}.has-small-font-size{font-size:var(--wp--preset--font-size--small) !important}.has-medium-font-size{font-size:var(--wp--preset--font-size--medium) !important}.has-large-font-size{font-size:var(--wp--preset--font-size--large) !important}.has-x-large-font-size{font-size:var(--wp--preset--font-size--x-large) !important}:root{--global-kb-font-size-sm:clamp(.8rem,.73rem + .217vw,.9rem);--global-kb-font-size-md:clamp(1.1rem,.995rem + .326vw,1.25rem);--global-kb-font-size-lg:clamp(1.75rem,1.576rem + .543vw,2rem);--global-kb-font-size-xl:clamp(2.25rem,1.728rem + 1.63vw,3rem);--global-kb-font-size-xxl:clamp(2.5rem,1.456rem + 3.26vw,4rem);--global-kb-font-size-xxxl:clamp(2.75rem,.489rem + 7.065vw,6rem)}:root{--global-palette1:#3182ce;--global-palette2:#2b6cb0;--global-palette3:#1a202c;--global-palette4:#2d3748;--global-palette5:#4a5568;--global-palette6:#718096;--global-palette7:#edf2f7;--global-palette8:#f7fafc;--global-palette9:#fff}div#section-subnav{margin-top:-65px}div#DivAssetPlaceHolder1{display:none}div#DivAssetPlaceHolder2{display:none}.wow{visibility:visible !important}img.gdpr-rt{display:none}.page-template-template-kadence-nowrapper .site-main{margin-top:0px}p.swp-result-item--desc{font-family:"Roboto",sans-serif;font-size:18px;font-weight:300;line-height:29px}#searchwp-form-1 .swp-input{border-radius:30px !important;background:unset}#searchModal #searchwp-form-1 .search-submit{color:transparent !important;background-color:transparent;background-image:url("data:image/svg+xml;charset=utf-8,%3Csvg xmlns='http://www.w3.org/2000/svg' width='15' height='15' fill='none'%3E%3Cpath fill='%23d9178d' d='M6.068 12.136c1.31 0 2.533-.426 3.527-1.136l3.74 3.74c.174.173.402.26.64.26.512 0 .883-.395.883-.9a.87.87 0 0 0-.253-.63L10.89 9.744a6.04 6.04 0 0 0 1.247-3.677C12.136 2.73 9.406 0 6.068 0 2.722 0 0 2.73 0 6.068s2.722 6.068 6.068 6.068m0-1.31c-2.612 0-4.758-2.154-4.758-4.758S3.456 1.31 6.068 1.31c2.604 0 4.758 2.154 4.758 4.758s-2.154 4.758-4.758 4.758'/%3E%3C/svg%3E");background-position:20px !important;background-repeat:no-repeat;background-size:40px auto}#searchModal #searchwp-form-1 .search-submit.nitro-lazy{background-image:none !important}.home p.swp-result-item--desc{-webkit-line-clamp:3;overflow:hidden;display:-webkit-box;-webkit-box-orient:vertical}.fixed-button{position:fixed;top:0px;right:30px;z-index:100}.admin-bar .fixed-button{top:30px}@media (max-width: 550px){.fixed-button{display:none}}.page-template-template-kadence-nowrapper button#rmp_menu_trigger-62790{display:none !important}.fixed-button a{background:#f7bf43 !important;border-top:2px solid #f7bf43 !important;border-bottom:2px solid #f7bf43 !important;border-left:2px solid #f7bf43 !important;border-right:2px solid #f7bf43 !important;color:#5e19eb !important}.fixed-button a:hover{background-color:#5e19eb !important;color:#f7bf43 !important}a.signmeup-button:hover{color:#f7bf43 !important}a.purple-button:hover{color:#5e19eb !important;border-top:1px solid #5e19eb !important;border-bottom:1px solid #5e19eb !important;border-left:1px solid #5e19eb !important;border-right:1px solid #5e19eb !important}a.green-button:hover{color:#7ed956 !important;border:none !important}a.orange-button:hover{border:none !important}a.gold-button:hover{border:none !important}.ha-event .entry-title a{color:#a20067 !important;font-size:18px !important;text-decoration:none !important}.ha-event__dates{font-size:18px !important}a.btn-nohover-purple,a.btn-nohover-purple:hover,a.btn-nohover-purple:focus,a.btn-nohover-purple:focus-visible,a.btn-nohover-purple:active,a.btn-nohover-white,a.btn-nohover-white:hover,a.btn-nohover-white:focus,a.btn-nohover-white:focus-visible,a.btn-nohover-white:active{box-shadow:none !important;outline:none !important;border-top:0 solid transparent !important;border-right:0 solid transparent !important;border-bottom:0 solid transparent !important;border-left:0 solid transparent !important;transition:none !important}a.btn-nohover-purple::before,a.btn-nohover-purple:hover::before,a.btn-nohover-purple:focus::before,a.btn-nohover-purple:focus-visible::before,a.btn-nohover-purple:active::before,a.btn-nohover-white::before,a.btn-nohover-white:hover::before,a.btn-nohover-white:focus::before,a.btn-nohover-white:focus-visible::before,a.btn-nohover-white:active::before{opacity:0 !important;background:transparent !important;transition:none !important}a.btn-nohover-purple,a.btn-nohover-purple:hover,a.btn-nohover-purple:focus,a.btn-nohover-purple:focus-visible,a.btn-nohover-purple:active{background-color:#5e19eb !important;color:#fff !important}a.btn-nohover-white,a.btn-nohover-white:hover,a.btn-nohover-white:focus,a.btn-nohover-white:focus-visible,a.btn-nohover-white:active{background-color:#fff !important;color:#5e19eb !important}.teal-gradient.kt-row-has-bg>.kt-row-layout-overlay{background:linear-gradient(to bottom,rgba(0,183,195,0) 0px,rgba(0,183,195,0) 380px,rgba(0,183,195,.1) 420px,rgba(0,183,195,.18) 540px,rgba(0,183,195,.55) 620px,rgba(0,183,195,.7) 720px,rgba(0,183,195,1) 100%) !important;-webkit-backdrop-filter:blur(2px);backdrop-filter:blur(2px);opacity:1 !important}#searchwp-form-1 .swp-input,#searchwp-form-1 .swp-select{border:1px solid #d9178d;border-radius:5px}#searchwp-form-1 .searchwp-form-input-container .swp-select{border-top-right-radius:0;border-bottom-right-radius:0;border-right:0}#searchwp-form-1 .searchwp-form-input-container .swp-select+.swp-input{border-top-left-radius:0;border-bottom-left-radius:0}#searchwp-form-1 input[type=submit]{border-radius:5px}#searchwp-form-1 .swp-toggle-checkbox:checked+.swp-toggle-switch,#searchwp-form-1 .swp-toggle-switch--checked{background:#d9178d}#searchwp-form-1 input[type=submit]{color:#d9178d}.su-spoiler.my-custom-spoiler>.su-spoiler-title{font-size:21px;background:#d9178d;color:#fff}

Antimicrobial resistance research

Support

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Talk with an expert