RNA sequencing analysis
Identify novel gene isoforms, determine gene fusions, annotate isoforms and alternative splicing events
Survey transcript diversity with easy-to-use Iso-Seq analysis
Using the long reads generated by Single Molecule, Real-Time (SMRT) sequencing, our Iso-Seq method provides reads that span entire transcript isoforms, eliminating the need for computational reconstruction, and enabling functional characterization of full-length transcript isoforms. Explore the RNA sequencing application and workflows.
RNA sequencing
Our Iso-Seq analysis empowers you to discover novel transcripts and genes, identify fusion genes, annotate isoforms and alternative splicing events.
SMRT Analysis
Iso-Seq analysis uses highly accurate long reads (HiFi reads) generated with the CCS algorithm to cluster and generate full-length, high-quality transcript isoforms. Isoforms can be then mapped to a reference. Iso-Seq analysis is an easy-to-use application accessible via the SMRT Link graphical user interface.
How it works
Full-length, HiFi reads (Q20, single-molecule resolution) are identified and clustered at the isoform level, then polished to create high-quality consensus.
Original publication
Gordon, S. P., et al. (2015). Widespread polycistronic transcripts in fungi revealed by single-molecule mRNA sequencing. PLoS One, 10(7), e0132628.
PacBio DevNet tools
There are several community-supported tools for extending the RNA analysis beyond isoform identification including annotation, comparison, ORF prediction, etc. They provide scientists with easy access to additional open-source algorithms such as:
SQANTI
In-depth characterization of isoforms obtained by full-length transcript sequencing
TAMA
Modular algorithms for transcript annotation
SMRT compatible analysis products
Our analysis partners offer several solutions for RNA analysis. Options include customized analysis based on project needs, full-service genomics analytics, cloud-based platforms with open source tools, or opportunity to run your own algorithms.
DNAnexus
Cloud-based genome informatics platform that provides data analysis tools and Iso-Seq analysis
Computomics
Custom genome analysis services including Iso-Seq analysis