Intron retention regulates STAT2 function and predicts immunotherapy response in lung cancer

Authors: Ryan P. Englander, Mattia Brugiolo, Te-Chia Wu, Mitch Kostich, Nathan K. Leclair, SungHee Park, Jacques Banchereau, Peter Yu, Andrew Salner, Romain Banchereau, Karolina Palucka, Olga Anczuków

The Iso-seq method doubled the number of isoforms detected compared to short-read RNA-seq, revealing splicing events with direct relevance to cancer immunotherapy. The authors note that long-read RNA sequencing “may hold the key to breakthroughs that lead to the next wave of therapeutic advances.” With current workflows using Kinnex kits and the Revio system, such insights can now be achieved at scale and reduced cost, requiring only half a SMRT Cell to generate equivalent data compared to 12 SMRT Cells in the study.

Journal: bioRxiv
DOI: 10.1101/2025.08.19.671121
Year: 2025

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