Background: Alterations of oral microbiota are the main cause of the progression of caries. The goal of this study was to characterize the oral microbiota in childhood caries based on single-molecule real-time sequencing. Methods: A total of 21 preschoolers, aged 3-5 years old with severe early childhood caries, and 20 age-matched, caries-free children as controls were recruited. Saliva samples were collected, followed by DNA extraction, Pacbio sequencing and phylogenetic analyses of the oral microbial communities. Results: 876 species derived from 13 known bacterial phyla and 110 genera were detected from 41 children using Pacbio sequencing. At the species level, 38 species, including Veillonella spp., Streptococcus spp., Prevotella spp. and Lactobacillus spp., showed higher abundance in the caries group compared to the caries-free group (p<0.05). the core microbiota at genus and species levels was more stable in caries-free micro-ecological niche. follow-up, oral examinations 6 months after sample collection, development of new dental caries observed 5 children (the transitional group) among 21 free children. compared with children, groups, species, which were abundant group, exhibited a relatively low abundance both group (p<0.05). we conclude that abiotrophia spp., neisseria spp. veillonella are essential for maintaining healthy microbial ecosystem. prevotella lactobacillus dialister filifactor may be related to pathogenesis progression caries.
Journal: Frontiers in microbiology
DOI: 10.3389/fmicb.2017.02244
Year: 2017