Single Molecule, Real-Time (SMRT®) Sequencing combines long reads with uniform coverage to provide uniquely comprehensive views of plant and animal genomes and transcriptomes. High-quality genome assemblies and evidence-based annotations promote improved genetic marker development, discovery of novel genes, and structural variation characterization.
The ability to identify and understand the functions of the complex microbial populations living in, on, and around us requires comprehensive characterization of each community member. Long reads, high accuracy, and single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, long-read metagenomic profiling, and shotgun metagenomic assembly.
To bring precision medicine to every patient, cancer researchers need a more comprehensive view of all the somatic variants in genes, transcripts and whole genomes that drive cancer biology. Single Molecule, Real-Time (SMRT) Sequencing delivers the read lengths, uniform coverage, and accuracy needed to access the complete size spectrum of driver mutations — from rare single nucleotide variants to complex structural variants. Full-length transcript sequencing brings clarity to tumor-specific isoform and splice variant expression, enabling the discovery of novel biomarkers for early detection, tumor stratification, treatment response, and drug resistance. With SMRT Sequencing, scientists gain new insight into the most…
SMRT Sequencing Is Smart Business: Scientists are relying on long-read sequencing for generating high-quality reference genomes, more accurate gene and transcript models, and an integrated view of the epigenome for their organisms of interest. Single Molecule, Real-Time (SMRT) Sequencing from PacBio is the most established and trusted long-read sequencing solution available today, delivering the most complete genomic information for microbes, complex plant and animal genomes, and human biomedical research challenges. Sequencing providers are rapidly adding SMRT Sequencing to their portfolios in order to meet their customers’ demand for long reads.
To understand the genetic factors underlying health and disease and to address hidden heritability, scientists require a more comprehensive view of all the variations in the human genome. Single Molecule, Real-Time (SMRT) Sequencing delivers the read lengths, uniform coverage, and accuracy needed for accessing the complete size spectrum of sequence variant types — from single nucleotides to complex structural variants. PacBio’s long single-molecule reads also provide direct variant phasing information across full-length genes and chromosome haplotype blocks. With SMRT Sequencing, scientists gain new insight into the genetic basis of health and disease.
The PacBio Platform includes an extensive software portfolio that employs key advantages of SMRT (Single Molecule, Real-Time) Sequencing technology: extraordinarily long reads, highest consensus accuracy, uniform coverage and simultaneous epigenetic characterization. Core elements of our analytical portfolio include SMRT Analysis software, DevNet and SMRT Compatible products.
Read our product brochure to learn more about how PacBio Systems and SMRT technology will accelerate your research by delivering the most comprehensive view of genomes, transcriptomes and epigenomes.
Uncovering a connection between genotype and phenotype requires examination of all the variant types in a genome. Single Molecule, Real-Time (SMRT) Sequencing provides contiguous long reads, high consensus accuracy, and uniform coverage opening up access to a broad range of structural variants (SV) across a wide range of sizes, types, and GC content.
The Iso-Seq method provides full-length cDNA sequences from the 5’ end of transcripts to their poly-A tails - eliminating the need for transcript reconstruction and inference - giving complete, unambiguous information about alternatively spliced exons, transcriptional start sites, and poly-adenylation sites. Confidently characterize the full complement of isoforms within targeted genes, or across an entire transcriptome.
Industrial microbiologists rely on comprehensive genomic information to identify and develop complex biological products. Single Molecule, Real-Time (SMRT) Sequencing delivers a more complete view of individual organisms and microbial communities, fueling research for modern pharmaceutical discovery, environmental remediation, chemical commodity production, and agriculture products.
Single Molecule, Real-Time (SMRT) Sequencing directly detects DNA modifications by measuring variation in the polymerase kinetics of DNA base incorporation during sequencing. With high throughput, long reads, and the sensitivity to detect epigenetic modification without amplification or chemical conversions, the PacBio Systems offer scalable solutions for assessing DNA modifications in bacterial and eukaryotic genomes.
Single Molecule Real-Time (SMRT) Sequencing delivers reads that span the lengths of the majority of HLA class I and II genes. Unambiguously phase 4-field HLA types without imputation. With a more accurate and complete picture, gain deeper understanding of immune-related disease causality, graft-versus-host disease in hematopoietic transplantation, and drug hypersensitivity.
The PacBio Systems combine single-molecule resolution, long reads, and uniform coverage, to fully characterize genetic complexity — including structural variants, indels, copy number variants, micro-satellites, somatic variants, haplotypes, and phasing. Move beyond simply cataloging SNPs and target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.
In order to understand the molecular mechanisms governing the outcomes of disease, health and survival, immunologists have to characterize exceptionally complex genomic regions, like major histocompatibility complex (MHC), killer cell immune receptors (KIR), and the B and T-cell immune repertoire. Single Molecule, Real-Time (SMRT) Sequencing delivers the long read lengths, uniform coverage and high accuracy necessary to comprehensively and confidently resolve these immune sub-genomic regions. The granularity of data generated by PacBio® reads provides new access to imputation-free characterization of genes and haplotypes for invaluable genomic insights to advance disease association and evolutionary research.