The Sequel System, powered by Single Molecule, Real Time (SMRT) Technology, delivers long reads, high consensus accuracy, uniform coverage and epigenetic characterization. This newly introduced platform provides higher throughput, more scalability, a reduced footprint and lower sequencing project costs compared to the PacBio RS, the original long-read sequencer.
To understand the genetic factors underlying health and disease and to address hidden heritability, scientists require a more comprehensive view of all the variations in the human genome. Single Molecule, Real-Time (SMRT) Sequencing delivers the read lengths, uniform coverage, and needed for accessing the complete size spectrum of sequence variant types — from single nucleotides to complex structural variants. PacBio’s long single-molecule reads also provide direct variant phasing information across full-length genes and chromosome haplotype blocks. With SMRT Sequencing, scientists gain new insight into the genetic basis of health and disease.
Single Molecule, Real-Time (SMRT) Sequencing combines long reads with uniform coverageto generate high-quality, contiguous assemblies. Comprehensive genomes and evidence-based annotations promote improved genetic marker development, the discovery of novelgenes, and structural variation characterization.
Single Molecule, Real-Time (SMRT) Sequencing offers affordable characterization of complete microbial genomes and populations. With this technology, scientists have the ability to simultaneously detail base modifications and mobile elements, quantify low-level variants, and achieve strain-level resolution within communities.
In order to understand the molecular mechanisms governing the outcomes of disease, health and survival, immunologists have to characterize exceptionally complex genomic regions, like major histocompatibility complex (MHC), killer cell immune receptors (KIR), and the B and T-cell immune repertoire. Single Molecule, Real-Time (SMRT) Sequencing delivers the long read lengths, uniform coverage and high accuracy necessary to comprehensively and confidently resolve these immune sub-genomic regions. The granularity of data generated by PacBio® reads provides new access to imputation-free characterization of genes and haplotypes for invaluable genomic insights to advance disease association and evolutionary research.
Single Molecule, Real-Time (SMRT) Sequencing delivers long reads with uniform coverage for the most comprehensive de novo genome assemblies generated today. True whole genome sequencing provides complete and accurate views of all types of genomic variation revealing structural variants, mobile elements, haplotypes, low complexity regions, and epigenetic modifications.
Single Molecule Real-Time (SMRT) Sequencing delivers reads that span the lengths of the majority of HLA class I and II genes. Unambiguously phase 4-field HLA types without imputation. With a more accurate and complete picture, gain deeper understanding of immune-related disease causality, graft-versus-host disease in hematopoietic transplantation, and drug hypersensitivity.
The PacBio Systems combine single-molecule resolution, long reads, and uniform coverage, to fully characterize genetic complexity — including structural variants, indels, copy number variants, micro-satellites, somatic variants, haplotypes, and phasing. Move beyond simply cataloging SNPs and target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.
Learn more about how SMRT Sequencing drives discovery in cancer research by providing the complete size spectrum of genetic variation in genes, transcripts, and whole genomes.
Single Molecule, Real-Time (SMRT) Sequencing directly detects DNA modifications by measuring variation in the polymerase kinetics of DNA base incorporation during sequencing. With high throughput, long reads, and the sensitivity to detect epigenetic modification without amplification or chemical conversions, the PacBio Systems offer scalable solutions for assessing DNA modifications in bacterial and eukaryotic genomes.
Scientists are relying on long-read sequencing more than ever before for generating high-quality reference genomes, more accurate gene and transcript models, and an integrated view of the epigenome of their organisms of interest. Single Molecule, Real-Time (SMRT) Sequencing from PacBio has rapidly expanded from the gold standard for microbial sequencing to a fully accepted and necessary sequencing solution for characterizing even the most complex plants and animals and for advanced human biomedical research. Long-read sequencing is becoming the norm for high-quality, complete genomes. Sequencing providers are adding SMRT Sequencing to their portfolios in order to meet this new demand and…
The ability to identify and understand the functions of the complex microbial populations living in, on, and around us requires comprehensive characterization of each community member. Long reads, high accuracy, and single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S sequencing, long-read metagenomic profiling, and shotgun metagenomic assembly.
Industrial microbiologists rely on comprehensive genomic information to identify and develop complex biological products. Single Molecule, Real-Time (SMRT) Sequencing delivers a more complete view of individual organisms and microbial communities, fueling research for modern pharmaceutical discovery, environmental remediation, chemical commodity production, and agriculture products.
The Iso-Seq method provides full-length cDNA sequences from the 5’ end of transcripts to their poly-A tails - eliminating the need for transcript reconstruction and inference - giving complete, unambiguous information about alternatively spliced exons, transcriptional start sites, and poly-adenylation sites. Confidently characterize the full complement of isoforms within targeted genes, or across an entire transcriptome.
Read our product brochure to learn more about how PacBio Systems and SMRT technology will accelerate your research by delivering the most comprehensive view of genomes, transcriptomes and epigenomes.