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Fine-tune your sequencing efforts with SMRT consumables

PacBio provides complete workflows and all the consumable components you need to perform Single Molecule, Real-Time (SMRT) Sequencing. Regardless of project size, our consumables promote customization while maintaining cost-effectiveness. Due to the flexibility of the PacBio sequencing platforms, these components support a variety of workflow steps and research goals. Long, accurate sequence reads are only a few steps away.

SMRT Sequencing workflows are:

  • Scalable
    • From short amplicons to large, complex genomes
  • Simple
    • From RNA or DNA to sequencing in about a day
  • Standardized
    • Similar for either the Sequel or PacBio RS II systems

Many of the workflow steps are similar between the Sequel and PacBio RS II systems. A typical workflow is described in the following figure.

Explore more detailed information about the consumables for each specific platform:

Sequel System consumables

PacBio RS II System consumables

The following is a general description of the SMRT Sequencing workflows and the types of samples you can analyze.


Prepare your sample, add polymerase, and go

PacBio protocols guide you through the steps to obtain sequence reads up to tens of thousands of base pairs long. In our template preparation steps, DNA is treated to ensure the removal of any DNA damage, and ends are repaired and circularized with the addition of SMRTbell adapters, as shown below. The result is a SMRTbell template. Any remaining non-circularized or nicked templates are digested with exonucleases.

The SMRTbell templates provide a segment of single-stranded DNA to which a sequencing primer is annealed. A polymerase is bound to a primer-annealed template and the complex is ready to be loaded onto SMRT Cells. The last step is important because we add a hairpin loop structure to each end of the template DNA, providing an optimized location to anneal the sequencing primer and bind a sequencing polymerase.

SMRTbell-template

 

 

Sequence a wide variety of sample types

SMRT technology can sequence essentially any DNA strand, whether it be short, long, high GC, or high AT. SMRT Sequencing typically uses non-amplified DNA. This allows you to resolve complex genetic challenges. For example, while sequencing the DNA, epigenetic information is collected at the same time as sequence information.

While our template preparation workflows typically start with double-stranded DNA, you can use RNA as your starting material with our Iso-Seq protocols. Using the Iso-Seq method, splice variants and many other novel aspects of full-length cDNA transcripts can be detected.

We offer several workflows to target specific regions of your genome of interest. Hybridization-based protocols allow you to focus your interests on multi-kilobase or megabase regions. For more focused regions, we offer solutions to efficiently multiplex amplicons up to 10 kb.

 

For more information about how our SMRT consumables can support your research, contact us.

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