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Industrial Biotechnology Brochure: Fuel biotech discovery with confident characterization of microbes and their communities

2016

Summary +

Industrial microbiologists rely on comprehensive genomic information to identify and develop complex biological products. Single Molecule, Real-Time (SMRT) Sequencing delivers a more complete view of individual organisms and microbial communities, fueling research for modern pharmaceutical discovery, environmental remediation, chemical commodity production, and agriculture products.

Microbial Communities Application Brochure: Complex microbial populations simplified

2016

Summary +

The ability to identify and understand the functions of the complex microbial populations living in, on, and around us requires comprehensive characterization of each community member. Long reads, high accuracy, and single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S sequencing, long-read metagenomic profiling, and shotgun metagenomic assembly.

Product Note: Fast, high-resolution DNA Sizing with the Fragment Analyzer system

2016

Summary +

The Fragment Analyzer instrument is a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accurately size fragments ranging from 10 bp up to 50 kb. This platform allows important DNA quality checkpoints to be completed in 1 hour for de novo large-genome sequencing projects and other PacBio applications leveraging multi-kilobase read lengths. The instrument can be used in place of time-consuming QC steps involving Pulsed Field Gel Electrophoresis, saving time by avoiding multiple overnight gel runs when preparing large-insert SMRTbell libraries. Alternative DNA-sizing instruments cannot accurately resolve large DNA fragments in this range.

Application Note: Multiplex target enrichment using barcoded multi-kilobase fragments and probe-based capture technologies

2016

Summary +

Target enrichment capture methods allow scientists to rapidly interrogate important genomic regions of interest for variant discovery, including SNPs, gene isoforms, and structural variation. Custom targeted sequencing panels are important for characterizing heterogeneous, complex diseases and uncovering the genetic basis of inherited traits with more uniform coverage when compared to PCR-based strategies. With the increasing availability of high-quality reference genomes, customized gene panels are readily designed with high specificity to capture genomic regions of interest, thus enabling scientists to expand their research scope from a single individual to larger cohort studies or population-wide investigations. Coupled with PacBio long-read sequencing, these technologies can capture 5 kb fragments of genomic DNA (gDNA), which are useful for interrogating intronic, exonic, and regulatory regions, characterizing complex structural variations, distinguishing between gene duplications and pseudogenes, and interpreting variant haplotyes. In addition, SMRT Sequencing offers the lowest GC-bias and can sequence through repetitive regions.

Application Notes: Targeted sequencing and chromosomal haplotype assembly using Cergentis TLA technology with SMRT Sequencing

2016

Summary +

The Targeted Locus Amplification (TLA) Technology from Cergentis enables the targeted, hypothesis-neutral, amplification of any genomic locus of interest over 50 kb using just one primer pair complementary to a short locus-specific sequence. TLA is a strategy to selectively amplify complete loci on the basis of crosslinking physically proximal sequences. Unlike other targeted sequencing methods, TLA works without prior detailed locus information, as one primer pair is sufficient to amplify tens to hundreds of kilobases of DNA surrounding that locus. In a separate application of TLA, the unamplified template can be used for genome-wide phasing and assembly. TLA enables targeted sequencing and detection of single nucleotide and structural variants in genes or regions of interest. SMRT sequencing enables end-to-end sequencing of multi-kilobase TLA amplicons or unamplified TLA templates. The combination of Cergentis' TLA and SMRT Sequencing technologies allows for sequencing and haplotyping of individual genes, chromosomes and genomes.

Epigenetics Application Brochure: Characterize the epigenetic landscape of your genome

2016

Summary +

Single Molecule, Real-Time (SMRT) Sequencing directly detects DNA modifications by measuring variation in the polymerase kinetics of DNA base incorporation during sequencing. With high throughput, long reads, and the sensitivity to detect epigenetic modification without amplification or chemical conversions, the PacBio Systems offer scalable solutions for assessing DNA modifications in bacterial and eukaryotic genomes.

Core Lab Brochure: Stand tall in a crowd of short-read providers

2016

Summary +

Scientists are relying on long-read sequencing more than ever before for generating high-quality reference genomes, more accurate gene and transcript models, and an integrated view of the epigenome of their organisms of interest. Single Molecule, Real-Time (SMRT) Sequencing from PacBio has rapidly expanded from the gold standard for microbial sequencing to a fully accepted and necessary sequencing solution for characterizing even the most complex plants and animals and for advanced human biomedical research. Long-read sequencing is becoming the norm for high-quality, complete genomes. Sequencing providers are adding SMRT Sequencing to their portfolios in order to meet this new demand and to differentiate services from their peers. SMRT Sequencing is the most popular, proven, and trusted long-read sequencing solution available today.

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