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Sequence entire genomes with uniform coverage

 

Most sequencing systems experience bias, which causes difficulties in sequencing AT-rich or GC-rich DNA regions, highly repetitive sequences, long homopolymers, and palindromic sequences. These challenging sequences often lead to incomplete results, sometimes missing as much as 15% of the genome.

Single Molecule, Real-Time (SMRT) Sequencing does not require an amplification step, leading to uniform coverage across all genomes. This allows you to sequence through palindromes and low-diversity regions of the genome. These long reads also allow for the spanning of complex regions.

Mean coverage per GC window

SMRT_Technology_Unbiased_Reads.Spotlight.Mean_Coverage_per_GC_window

Scientists analyzed the GC content of the human CHM1 genome by coverage levels. Their data demonstrates the uniform coverage of SMRT Sequencing1.

Contact us for more information about incorporating SMRT Sequencing into your research efforts.

Reference

  1. Chaisson, M., et al., (2015) Resolving the complexity of the human genome using single-molecule sequencing. Nature. 517 (7536), 608–611.

 

Selected Resources