Sequence entire genomes with uniform coverage
Most sequencing systems experience bias, which causes difficulties in sequencing AT-rich or GC-rich DNA regions, highly repetitive sequences, long homopolymers, and palindromic sequences. These challenging sequences often lead to incomplete results, sometimes missing as much as 15% of the genome.
Single Molecule, Real-Time (SMRT) Sequencing does not require an amplification step, leading to uniform coverage across all genomes. This allows you to sequence through palindromes and low-diversity regions of the genome. These long reads also allow for the spanning of complex regions.
Mean coverage per GC window
Scientists analyzed the GC content of the human CHM1 genome by coverage levels. Their data demonstrates the uniform coverage of SMRT Sequencing1.
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Chaisson, M., et al., (2015) Resolving the complexity of the human genome using single-molecule sequencing. Nature. 517 (7536), 608–611.
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature Reviews. Genetics
- Koren, Sergey et al. (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Current Opinion in Microbiology
- Chaisson, Mark J P et al. (2015) Resolving the complexity of the human genome using single-molecule sequencing. Nature
- Chin, Chen-Shan et al. (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nature Methods
- Ross, Michael G et al. (2013) Characterizing and measuring bias in sequence data. Genome Biology
- Jonas Korlach et al. (2014) Returning to more finished genomes Genomics Data
- Ashley, Euan (2016) AGBT Roche and PacBio Workshop: Towards precision medicine
- Gyllensten, Ulf (2015) AGBT Virtual Poster: Clinical sequencing using Pacific Biosciences RS II for HLA typing and monitoring of drug resistance in chronic myeloid leukemia (CML)
- Korlach, Jonas (2016) Mendelspod: A home run on the first hit – PacBio’s Jonas Korlach
- Korlach, Jonas (2014) Seminar: Gain new insights in genome and transcriptome research with >10,000 bp reads