Produce exceptional results with long reads
As the field of genomics evolves, there is a growing awareness in the scientific community of the importance of long-read data. Long sequence reads improve mappability for resequencing and simplify de novo assembly. PacBio Systems allow you to directly sequence DNA and achieve long sequencing reads with uniform coverage. Our Single Molecule, Real-Time (SMRT) Sequencing technology consistently produces some of the longest average read lengths available in the industry (average > 10,000 bp, some reads > 60,000 bp).
These long reads give you the ability to:
- Assemble high-quality de novo genomes
- Catalog full-length isoforms
- Unambiguously align sequences
- Observe fully phased alleles
- Span repetitive elements and complex regions
- Resolve structural variants
Read length data shown above from a 30 kb size-selected human library on the Sequel System (10-hour movie, 2.0 chemistry) with a total output of 7.6 Gb. Each Sequel System SMRT Cell 1M generates ~365,000 reads.
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Chin, Chen-Shan et al. (2016) Phased diploid genome assembly with Single Molecule Real-Time Sequencing bioRxiv
- Huang, Da Wei et al. (2016) Towards better precision medicine: PacBio Single-Molecule long reads resolve the interpretation of HIV drug resistant mutation profiles at explicit quasispecies (haplotype) level. Journal of Data Mining in Genomics & Proteomics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature Biotechnology
- Koren, Sergey et al. (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Current Opinion in Microbiology
- Chaisson, Mark J P et al. (2015) Resolving the complexity of the human genome using single-molecule sequencing. Nature
- Treutlein, Barbara et al. (2014) Cartography of neurexin alternative splicing mapped by single-molecule long-read mRNA sequencing. Proceedings of the National Academy of Sciences
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome Research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biology
- Chin, Chen-Shan et al. (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nature Methods
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, Proteomics & Bioinformatics
- Jonas Korlach et al. (2014) Returning to more finished genomes Genomics Data
- Giallonardo, Francesca Di et al. (2014) Full-length haplotype reconstruction to infer the structure of heterogeneous virus populations. Nucleic Acids Research
- Poster: Hall, Richard J. et al. (2015) Assembly of complete KIR haplotypes from a diploid individual by the direct sequencing of full-length fosmids.
- Presentation: Schatz, Michael et al. (2015) The resurgence of reference quality genome sequence.
- Poster: Clark, Tyson A et al. (2015) Single Molecule, Real-Time sequencing of full-length cDNA transcripts uncovers novel alternatively spliced isoforms.
- Ashley, Euan (2016) AGBT Roche and PacBio Workshop: Towards precision medicine
- Gyllensten, Ulf (2015) AGBT Virtual Poster: Clinical sequencing using Pacific Biosciences RS II for HLA typing and monitoring of drug resistance in chronic myeloid leukemia (CML)
- Korlach, Jonas (2016) Mendelspod: A home run on the first hit – PacBio’s Jonas Korlach
- Korlach, Jonas (2014) Seminar: Gain new insights in genome and transcriptome research with >10,000 bp reads
- Sebra, Robert (2015) Customer Experience: At Mount Sinai School of Medicine, sequence reads longer than 70 kb