We have designed PacBio technologies to focus on efficiency, and we constantly strive to deliver more effective sequencing runs. To further these efforts, we have selected experienced partners that focus specifically on critical aspects of the sequencing workflow. These partners offer services in the following areas:
- Nucleic acid extraction
- cDNA synthesis
- Size selection
- Target enrichment
- Template-prep automation
- Quality-control instrumentation
NUCLEIC ACID EXTRACTION
For more information about nucleic acid extraction, please see “Recommended DNA Isolation Kits” in the Template Preparation and Sequencing Guide.
SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications, including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantage of these known sequences, the simplicity and efficiency of the single-step SMART process permits unparalleled sensitivity, and ensures that full-length cDNA is generated and amplified.
Clontech Laboratories, Inc.
Home page: http://www.clontech.com/
Product page: http://www.clontech.com/US/Products/cDNA_Synthesis_and_Library_Construction/cDNA_Synthesis_Kits/SMARTer_Kits?sitex=10020:22372:US
Customer support email: email@example.com
Customer support phone: 800.662.2566
SHEARING & FRAGMENTATION
g-TUBE, the latest innovation from Covaris, is a single-use consumable that enables scientists to shear genomic DNA into selected fragments sizes ranging from 6 kbp to 20 kbp. The only equipment needed is a compatible bench-top centrifuge and a pipette. g-TUBE uses centrifugal force (the “g” in g-TUBE) to push the sample through a precisely manufactured orifice in the embedded ruby. This produces shearing forces in the sample that fragment the DNA. Fragment size is selected by adjusting the centrifuge rotor speed, which alters the flow rate through the ruby and creates shearing forces. Higher centrifugation RPM will produce shorter fragments, and the protocol provided by Covaris contains the settings needed to select a fragment size between 6 kbp and 20 kbp. Remarkably, the entire process takes only 3 minutes, and up to 24 samples can be processed simultaneously.
Home page: http://www.covarisinc.com/
g-TUBE product page: http://covarisinc.com/products/g-tube/
Customer support email: CustomerService@covarisinc.com
Customer support phone: +1.781.932.3959
Diagenode is a leading global provider of complete solutions for epigenetics research, biological sample preparation, and diagnostics assays. The company offers the Megaruptor® shearing instrument, which was designed to provide researchers with a simple, automated, and highly reproducible device for long fragment DNA shearing from 3kb – 75kb. The shearing performance of the Megaruptor is independent of the source, concentration, temperature, or salt content of a DNA sample. The Megaruptor shears DNA fragments above 20kb, which are required for the construction of ultra-long libraries prior to sequencing on the instrument.
Megaruptor genomic DNA shearing for large insert sequencing library preparation
Application of the Megaruptor for shearing of ultra-long DNA fragments for third generation sequencing technologies
Home page: https://www.diagenode.com/
Product page: https://www.diagenode.com/p/megaruptor2-1-unit
Customer support email:
North America: firstname.lastname@example.org
Sage Science develops products for the life sciences research markets. Their mission is to provide new systems to streamline and improve sample-preparation workflows for DNA and protein analysis. Among those products are the:
The BluePippin has pulsed-field electrophoresis capabilities for resolving and collecting high molecular weight DNA up to 50 kb. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to five samples/gel cassette may be run, with no possibility of cross contamination.
PippinHT incorporates the company’s highly regarded sizing technology, and increases throughput to 24 samples per run, compared to a maximum of 5 with BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing large-scale sequencing data.
The SageELF is an electrophoresis system that separates DNA or protein samples by size, and then fractionates the whole sample, or section of sample, into 12 fractions. The system is equipped with pulsed-field electrophoresis for resolving large DNA. Fractionation ranges are estimated and adjusted in software, and fractions are collected in buffer. One sample is fractionated on a single precast agarose cassette, and one or two cassettes may be processed at one time.
Sage Science, Inc.
Home page: www.sagescience.com
Products page: http://www.sagescience.com/products/
Customer support email: email@example.com
Customer support phone: 888.744.0144
Now you can amplify highly polymorphic HLA genes with confidence and analyze both alleles in a heterozygous individual using the PacBio System. The product line includes HLA Class I and HLA Class II typing kits. Whole HLA genes are amplified with NGSgo‐AmpX amplification primers in a single reaction using QIAGEN LongRange polymerase. DNA template prep and related sequencing reagents are purchased directly from PacBio. The amplicons generated by the NGSgo‐AmpX kit are ready to go directly into barcoded adapter SMRTbell library preparation and subsequent SMRT Sequencing. The resulting PacBio consensus sequences are fully compatible with the NGSengine analysis software.
Genome Diagnostics B.V.
Home page: http://www.gendx.com/home
Product page: http://www.gendx.com/pacbio-ngs
Customer support phone: +18.104.22.168.3799
Customer support email: firstname.lastname@example.org
xGen® target capture products from IDT enable greater sensitivity and higher sample throughput for targeted next generation sequencing (NGS). NGS researchers can now benefit from the IDT high throughput, quality controlled manufacturing process to order gene panels that have the greatest level of uniformity. These panels can be easily optimized with the addition of more probes. xGen Lockdown Probes are individually synthesized oligonucleotides that offer an unprecedented level of flexibility and scalability. The probes, which are available at different scales, are assessed by mass spectrometry for quality control, enabling a seamless transition from discovery to clinical application.
Integrated DNA Technologies, Inc.
Home page: https://www.idtdna.com/site
Product page: https://www.idtdna.com/pages/products/nextgen/target-capture
Customer support phone: 1-800-328-2661
Customer support email: email@example.com
SeqCap EZ System is a solution-based capture system that enables enrichment of the whole exome or targeted customer regions of interest in a single test tube. Built upon an optimized design algorithm, SeqCap EZ Library sets a new standard for a simple single-step enrichment method.
Roche NimbleGen, Inc.
Home page: http://sequencing.roche.com/
Product page: http://www.nimblegen.com/products/seqcap/ez-system/index.html
Customer support phone: 800.262.1640
Customer support email: firstname.lastname@example.org
The NGS Workstation (Option B) offers the advantage of increasing throughput without affecting data quality, freeing up researchers from performing time consuming and repetitive tasks. With plate and tip handling solutions, the workstation also enables walk away automation, so researchers have time for valuable tasks.
Application note: Automation of PacBio SMRTbell 10 kb Template Preparation on an Agilent NGS Workstation
Home page: https://www.agilent.com/home
Product page: https://www.chem.agilent.com/en-US/products-services/Instruments-Systems/Automation-Solutions/NGS-Workstation-Option-B/Pages/default.aspx
Customer support phone: 1.800.979.4811
Customer support email: email@example.com
The Sciclone NGS (Next Generation Sequencing) Workstation (P/N SG3-31020-0300) is the most capable automated solution for high-throughput sequencing sample preparation. Built on the Sciclone G3 platform, the NGS Workstation is a complete benchtop solution for library prep, sequence capture, and normalization.
Perkin Elmer, Inc.
Home page: http://www.perkinelmer.com/corporate/
Product page: http://www.perkinelmer.com/Catalog/Family/ID/Sciclone%20NGS%20Workstation
Customer support phone: +1.800.762.4000
Customer support email: CustomerCareUS@perkinelmer.com
The Fragment Analyzer™ instrument offers a fast, high-resolution benchtop capillary electrophoresis (CE) platform that utilizes proprietary markers to accurately size fragments ranging from 10 bp up to 50 Kb. The platform allows important DNA quality checkpoints to be completed in 1 hour, saving time by avoiding multiple overnight gel runs when preparing large-insert SMRTbell libraries.
Product note: Fast, High Resolution DNA Sizing with the Fragment Analyzer System
Home page: http://www.aati-us.com/
Product page: http://www.aati-us.com/product/fragment-analyzer
Customer support phone: 515.964.8500
Customer support email: firstname.lastname@example.org
For more information on quality-control instrumentation, please see “Specific Lab Equipment and Related Consumables Required” in the Template Preparation and Sequencing Guide.