The gateway to new biological discoveries
Epigenetic modifications affect a broad range of biological processes, including gene expression, host-pathogen interactions, environmental response, DNA damage, and DNA repair1. Epigenetics also play a significant role in the inheritance of traits from one generation to the next. While more than 20 types of epigenetic and DNA-damage modifications have been identified, most scientists have only been able to study one type, cytosine methylation, using indirect detection methods. The ability to directly detect these changes is critical for understanding the impact of DNA modification on a wide variety of natural processes and disease responses.
Characterize the epigenetic landscape of your genome
Single Molecule, Real-Time (SMRT) Sequencing directly detects epigenetic modifications by measuring kinetic variation during base incorporation. By capturing these modifications simultaneously with sequence data, this method eliminates the need for special sample preparation and additional sequencing.
PacBio Systems provide a direct view of epigenetic modification
To learn more about how SMRT Sequencing simultaneously captures genomic and epigenetic information, contact us.
- Davis, B. M., et al., (2013) Entering the era of bacterial epigenomics with single molecule real time DNA sequencing. Current Opinion in Microbiology. 16 (2), 192–198.
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- Greer, Eric Lieberman et al. (2015) DNA methylation on N6-adenine in C. elegans. Cell
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- Ouellette, Matthew et al. (2015) Genome-wide DNA methylation analysis of Haloferax volcanii H26 and identification of DNA methyltransferase related PD-(D/E)XK nuclease family protein HVO_A0006. Frontiers in Microbiology
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- Feng, Z et al. (2015) qDNAmod: a statistical model-based tool to reveal intercellular heterogeneity of DNA modification from SMRT sequencing data. Nucleic Acids Research
- O'Loughlin, Jason L et al. (2015) Analysis of the Campylobacter jejuni genome by SMRT DNA Sequencing identifies restriction-modification motifs. PLoS One
- Roberts, Richard J et al. (2015) REBASE–a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Research
- Goldfarb, Tamara et al. (2015) BREX is a novel phage resistance system widespread in microbial genomes. The EMBO Journal
- Poster: Korlach, J. et al. (2015) Epigenome characterization of human genomes using the PacBio platform
- Poster: Korlach, Jonas et al. (2015) Whole genome sequencing and epigenome characterization of cancer cells using the PacBio platform.
- Poster: Hall, Richard J et al. (2015) A workflow for the analysis of contigs from the metagenomic shotgun assembly of SMRT Sequencing data
- Conference Presentation: Korlach, Jonas (2015) ASHG Workshop Presentation: Going beyond the $1,000 genome?: the future of high quality de novo human genomes, epigenomes and transcriptomes?
- Brochures: Bring the “W” back to Whole genome sequencing (2015)
- Brochures: Sequel System – Introducing the scalable platform for SMRT Sequencing (2015)
- Brochures: Gain a deeper understanding of your sequencing data (2015)
- Brochures: Revolutionize genomics with SMRT Sequencing (2015)
- Brochures: Novel insights into microbial and viral complexity. (2015)
- Service Provider and Core Lab Profiles: Long reads yield complete microbial genomes and improved large genome assemblies at the Institute for Genome Sciences. (2015)