Characterize complex populations with single-molecule resolution
Distinguishing closely related DNA molecules in the same sample requires single-molecule resolution with high accuracy and long read lengths. For these applications, we have developed a sequencing mode that generates intra-molecular consensus called Circular Consensus Sequencing (CCS).
Single Molecule, Real-Time (SMRT) Sequencing is the only technology that generates this level of single-molecule consensus accuracy at multi-kilobase read lengths, and it has been used for a variety of complex population applications. With this advanced sequencing method, you have the ability to:
- Deconvolute complex mixtures of unique haplotypes
- Accurately identify somatic variants
- Resolve complex communities
The circular nature of the SMRTbell DNA template allows polymerase to sequence the same DNA molecule multiple times with multiple passes. This produces high intra-molecular consensus accuracy.
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Hebert, Paul DN et al. (2017) A sequel to Sanger: amplicon sequencing that scales bioRxiv
- Pootakham, Wirulda et al. (2017) High resolution profiling of coral-associated bacterial communities using full-length 16S rRNA sequence data from PacBio SMRT sequencing system. Scientific Reports
- Motooka, Daisuke et al. (2017) Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese individuals. Frontiers in Microbiology
- Potapov, Vladimir et al. (2017) Examining sources of error in PCR by single-molecule sequencing. PLoS One
- Laird Smith, Melissa et al. (2016) Rapid sequencing of complete env genes from primary HIV-1 samples Virus Evolution
- Zhang, Huibin et al. (2016) Comprehensive mutagenesis of the fimS promoter regulatory switch reveals novel regulation of type 1 pili in uropathogenic Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America
- Huang, Da Wei et al. (2016) Towards better precision medicine: PacBio single-molecule long reads resolve the interpretation of HIV drug resistant mutation profiles at explicit quasispecies (haplotype) level. Journal of Data Mining in Genomics & Proteomics
- Russo, Giancarlo et al. (2015) Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing. Applied & Translational Genomics
- Westbrook, Catherine J et al. (2015) No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing. Human Immunology
- Guo, Xiaoge et al. (2015) SMRT Sequencing for parallel analysis of multiple targets and accurate SNP phasing. G3
- Roberts, Richard J et al. (2013) The advantages of SMRT sequencing. Genome Biology
- Peccoud, Jean et al. (2017) Untangling heteroplasmy, structure, and evolution of an atypical mitochondrial genome by PacBio Sequencing. Genetics
- Hestand, Matthew S et al. (2016) Polymerase specific error rates and profiles identified by single molecule sequencing. Mutation Research
- Giallonardo, Francesca Di et al. (2014) Full-length haplotype reconstruction to infer the structure of heterogeneous virus populations. Nucleic Acids Research
- Travers, Kevin J et al. (2010) A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic Acids Research
- Poster: Ekholm, J. et al. (2016) Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Poster: Laird Smith, M. et al. (2016) An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)
- Poster: Heiner, C. et al. (2016) WGS SMRT Sequencing of patient samples from a fecal microbiota transplant trial
- Poster: Hepler, N. Lance et al. (2016) An improved circular consensus algorithm with an application to detection of HIV-1 Drug-Resistance Associated Mutations (DRAMs)
- Poster: Sethuraman, Anand et al. (2015) Analysis of full-length metagenomic 16S genes by Single Molecule, Real-Time Sequencing
- Poster: Hall, Richard J. et al. (2015) Profiling metagenomic communities using circular consensus and Single Molecule, Real-Time Sequencing.
- Presentation: Alexander, David et al. (2015) PacBio SMRT Analysis 3.0 preview
- Poster: Russo, Giancarlo et al. (2014) Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing.
- Ekholm, J. and Tsai, Y. and Greenberg, D. and Clark, T. (2016) ASHG Virtual Poster: Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Paxinos, Ellen (2016) AGBT Virtual Poster: An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)