Gain a full view of genomes with simultaneous epigenetic characterization
Given the central role of epigenetics in a wide variety of natural and disease processes, the ability to detect these and other changes is key to a more complete understanding of biological complexity. With Single Molecule, Real-Time (SMRT) Sequencing and software, researchers can automatically detect DNA modifications and measure the rate of DNA base incorporation during sequencing.
Epigenetic characterization adds depth to genetic research and analysis by offering the ability to:
- Find methyltransferase recognition motifs throughout a bacterial genome
- Identify locations of adenine and cytosine methylation
- Detect integrated sequences and chemical modifications
- Resolve strand-specific modifications
- Perform hypothesis-free detection
DNA polymerization runs freely at ~3 bases/second. Alteration of this rate due to the incorporation of nucleotides across modified bases is detected and used to infer the presence of bases other than A, C, T or G. This information is automatically generated and processed during every run.
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Suzuki, Yuta et al. (2016) AgIn: Measuring the landscape of CpG methylation of individual repetitive elements. Bioinformatics
- Koren, Sergey et al. (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Current Opinion in Microbiology
- Roberts, Richard J et al. (2015) REBASE–a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Research
- Roberts, Richard J et al. (2013) The advantages of SMRT sequencing. Genome Biology
- Korlach, Jonas et al. (2012) Going beyond five bases in DNA sequencing. Current Opinion in Structural Biology
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature Methods
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, Proteomics & Bioinformatics
- Jonas Korlach et al. (2014) Returning to more finished genomes Genomics Data
- Clark, Tyson A et al. (2011) Direct detection and sequencing of damaged DNA bases. Genome Integrity
- Poster: Ekholm, J. et al. (2016) Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Ekholm, J. and Tsai, Y. and Greenberg, D. and Clark, T. (2016) ASHG Virtual Poster: Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Korlach, Jonas (2014) Seminar: Gain new insights in genome and transcriptome research with >10,000 bp reads
- Clark, Tyson (2012) AGBT Virtual Poster: Detection of damaged DNA bases using SMRT Sequencing