Advance genomics with Single Molecule, Real-Time (SMRT) Sequencing
In an effort to overcome inherent challenges in the field of genomics, we sought to develop novel technology that pushed the boundaries of sequencing. The result, SMRT Sequencing, harnesses the natural process of DNA replication and enables real-time observation of DNA synthesis. With this unique technology, we equip innovative scientists and deliver the results needed to drive genetic discovery.
SMRT Sequencing is built upon two key innovations: zero-mode waveguides (ZMWs) and phospholinked nucleotides. ZMWs allow light to illuminate only the bottom of a well in which a DNA polymerase/template complex is immobilized. Phospholinked nucleotides allow observation of the immobilized complex as the DNA polymerase produces a completely natural DNA strand.
The SMRT Sequencing advantage
SMRT Sequencing is ideal for a variety of research applications and offers many benefits, including:
- Longest average read lengths
- Highest consensus accuracy
- Uniform coverage
- Simultaneous epigenetic characterization
- Single-molecule resolution
An overview of SMRT Sequencing
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
- Russo, Giancarlo et al. (2015) Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing. Applied & Translational Genomics
- Guo, Xiaoge et al. (2015) SMRT Sequencing for parallel analysis of multiple targets and accurate SNP phasing. G3
- Westbrook, Catherine J et al. (2015) No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing. Human Immunology
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature Reviews. Genetics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature Biotechnology
- Koren, Sergey et al. (2015) One chromosome, one contig: complete microbial genomes from long-read sequencing and assembly. Current Opinion in Microbiology
- Chaisson, Mark J P et al. (2015) Resolving the complexity of the human genome using single-molecule sequencing. Nature
- Roberts, Richard J et al. (2015) REBASE–a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Research
- Treutlein, Barbara et al. (2014) Cartography of neurexin alternative splicing mapped by single-molecule long-read mRNA sequencing. Proceedings of the National Academy of Sciences
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome Research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biology
- Roberts, Richard J et al. (2013) The advantages of SMRT sequencing. Genome Biology
- Chin, Chen-Shan et al. (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nature Methods
- Ross, Michael G et al. (2013) Characterizing and measuring bias in sequence data. Genome Biology
- Korlach, Jonas et al. (2012) Going beyond five bases in DNA sequencing. Current Opinion in Structural Biology
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature Methods
- Presentation: Alexander, David et al. (2015) PacBio SMRT Analysis 3.0 preview
- Poster: Hall, Richard J. et al. (2015) Profiling metagenomic communities using circular consensus and Single Molecule, Real-Time Sequencing.
- Poster: Sethuraman, Anand et al. (2015) Analysis of full-length metagenomic 16S genes by Single Molecule, Real-Time Sequencing
- Poster: Hall, Richard J. et al. (2015) Assembly of complete KIR haplotypes from a diploid individual by the direct sequencing of full-length fosmids.
- Poster: Brown, Michael et al. (2015) High-accuracy, single-base resolution of near-full-length HIV genomes.
- Presentation: Schatz, Michael et al. (2015) The resurgence of reference quality genome sequence.
- Poster: Clark, Tyson A et al. (2015) Single Molecule, Real-Time sequencing of full-length cDNA transcripts uncovers novel alternatively spliced isoforms.
- Gyllensten, Ulf (2015) AGBT Virtual Poster: Clinical sequencing using Pacific Biosciences RS II for HLA typing and monitoring of drug resistance in chronic myeloid leukemia (CML)
- Isoform Sequencing Application Brochure: Read full-length transcripts – no assembly required (2015)
- Sequel System Brochure: Introducing the scalable platform for SMRT Sequencing (2015)
- HLA Sequencing Application Brochure: Fully phased, allele-specific HLA sequencing – the perfect pair (2015)
- Targeted Sequencing Application Brochure: Capture your regions of interest in high resolution (2015)
- De Novo Assembly Application Brochure: Bring the “W” back to Whole genome sequencing (2015)