Advance genomics with Single Molecule, Real-Time (SMRT) Sequencing
In an effort to overcome inherent challenges in the field of genomics, we sought to develop novel technology that pushed the boundaries of sequencing. The result, SMRT Sequencing, harnesses the natural process of DNA replication and enables real-time observation of DNA synthesis. With this unique technology, we equip innovative scientists and deliver the results needed to drive genetic discovery.
SMRT Sequencing is built upon two key innovations: zero-mode waveguides (ZMWs) and phospholinked nucleotides. ZMWs allow light to illuminate only the bottom of a well in which a DNA polymerase/template complex is immobilized. Phospholinked nucleotides allow observation of the immobilized complex as the DNA polymerase produces a completely natural DNA strand.
The SMRT Sequencing advantage
SMRT Sequencing is ideal for a variety of research applications and offers many benefits, including:
- Longest average read lengths
- Highest consensus accuracy
- Uniform coverage
- Simultaneous epigenetic characterization
- Single-molecule resolution
An overview of SMRT Sequencing
Contact us for more information about incorporating SMRT Sequencing into your research efforts.
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- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature Methods
- Poster: Ekholm, J. et al. (2016) Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Poster: Laird Smith, M. et al. (2016) An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)
- Poster: Heiner, C. et al. (2016) WGS SMRT Sequencing of patient samples from a fecal microbiota transplant trial
- Poster: Sethuraman, Anand et al. (2016) Highly sensitive and cost-effective detection of somatic cancer variants using single-molecule, real-time sequencing
- Poster: Hepler, N. Lance et al. (2016) An improved circular consensus algorithm with an application to detection of HIV-1 Drug-Resistance Associated Mutations (DRAMs)
- Presentation: Alexander, David et al. (2015) PacBio SMRT Analysis 3.0 preview
- Poster: Sethuraman, Anand et al. (2015) Analysis of full-length metagenomic 16S genes by Single Molecule, Real-Time Sequencing
- Poster: Hall, Richard J. et al. (2015) Profiling metagenomic communities using circular consensus and Single Molecule, Real-Time Sequencing.
- Poster: Hall, Richard J. et al. (2015) Assembly of complete KIR haplotypes from a diploid individual by the direct sequencing of full-length fosmids.
- Poster: Brown, Michael et al. (2015) High-accuracy, single-base resolution of near-full-length HIV genomes.
- Presentation: Schatz, Michael et al. (2015) The resurgence of reference quality genome sequence.
- Poster: Clark, Tyson A et al. (2015) Single Molecule, Real-Time sequencing of full-length cDNA transcripts uncovers novel alternatively spliced isoforms.
- Ashley, Euan (2016) ASHG PacBio Workshop: Towards precision medicine
- Ekholm, J. and Tsai, Y. and Greenberg, D. and Clark, T. (2016) ASHG Virtual Poster: Enrichment of unamplified DNA and long-read SMRT Sequencing to unlock repeat expansion disorders
- Ashley, Euan (2016) AGBT Roche and PacBio Workshop: Towards precision medicine
- Paxinos, Ellen (2016) AGBT Virtual Poster: An improved circular consensus algorithm with an application to detect HIV-1 Drug Resistance Associated Mutations (DRAMs)
- Gyllensten, Ulf (2015) AGBT Virtual Poster: Clinical sequencing using Pacific Biosciences RS II for HLA typing and monitoring of drug resistance in chronic myeloid leukemia (CML)
- SMRT Sequencing Brochure: Revolutionize genomics with SMRT Sequencing (2017)
- De Novo Assembly Application Brochure: Bring the “W” back to Whole genome sequencing (2015)
- HLA Sequencing Application Brochure: Fully phased, allele-specific HLA sequencing – the perfect pair (2015)
- Sequel System Brochure: Introducing the scalable platform for SMRT Sequencing (2015)
- Targeted Sequencing Application Brochure: Capture your regions of interest in high resolution (2015)