Targeted sequencing experiments commonly rely on either PCR or hybrid capture to enrich for targets of interest. When using short read sequencing platforms, these amplicons or fragments are frequently targeted to a few hundred base pairs to accommodate the read lengths of the platform. Given PacBio’s long readlength, it is straightforward to sequence amplicons or captured fragments that are multiple kilobases in length. These long sequences are useful for easily visualizing variants that include SNPs, CNVs and other structural variants, often without assembly. We will review methods for the sequencing of long amplicons and provide examples using amplicons that range from several kilobases up to 17 kilobases. In addition, we will review methods for the capture of fragments longer than 5 kb using hybrid capture technologies and show an example in which a panel of 35 Alzheimer’s Disease genes are captured.
February 5, 2021 | Webinar