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PAG Conference: Sequencing and assembly of the rice variety N22 (aus group) – A new reference genome to study comparative, evolutionary and functional genomics of rice

David Kudrna, Rod Wing, and the Arizona Genomics Institute (AGI) plan to fully sequence and annotate the genomes and transcriptomes of 3-4 accessions from each of the estimated 9-15 subpopulation of rice. These subpopulation-specific references will be used to map resequencing data of 3,000 individuals for variation discovery, GWAS, and genomic selection studies to address important traits such as biotic and abiotic stress tolerances, yield, and grain quality. Here Dr. Kudrna presents the first high-quality genome sequence of the rice variety Nagina22. AGI produced and assembled 65-fold coverage of SMRT Sequencing data, resulting in an assembly of 373 Mb with a contig N50 of 906 kb and the longest contig 3.5 Mb. This and the planned additional high-quality reference genome assemblies will enable characterization and classification of the unlocked genetic potential of this vital crop, facilitating the next generation of green super crops to feed the world.

 

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