We are excited to announce our newest Single Molecule, Real-Time sequencer, the Sequel™ System. Watch this short video to learn about this exciting evolution in SMRT® Sequencing. The Sequel System provides higher throughput, more scalability, a reduced footprint and lower sequencing project costs compared to the PacBio® RS II System, while maintaining the benefits of SMRT technology. The core of the Sequel System is the capacity of its redesigned SMRT Cells, which contain one million zero-mode waveguides (ZMWs) at launch, compared to 150,000 ZMWs in the PacBio RS II. Active individual polymerases are immobilized within the ZMWs, providing windows to…
We are proud to announce the introduction of several new solutions for targeted sequencing and sample multiplexing on the PacBio® Sequencing System. New Targeted Sequencing Workflow through Collaboration with Roche NimbleGen Today we announced a new workflow that combines Roche NimbleGen’s SeqCap EZ enrichment technology with large DNA fragments (up to 6 kb) and our Single Molecule, Real-Time (SMRT®) Sequencing to provide a more comprehensive view of variants, transgene integration sites, and haplotype information over multi-kilobase contiguous regions. The laboratory workflow is described in a shared protocol. For each targeted region, SAMtools are used to phase and bin reads by…
We are pleased to announce the launch of our new reagent kit, P6-C4, which represents the next generation of our polymerase as well as our chemistry. This kit replaces the P5-C3 chemistry and is recommended for all SMRT® Sequencing applications, including de novo assembly, targeted sequencing, isoform sequencing, minor variant detection, scaffolding, long-repeat spanning, SNP phasing, and structural variant analysis. P6-C4 continues the steady read length improvement our users have seen since the instrument first launched. With this new chemistry, average read lengths increase to 10 kb - 15 kb, with half of all data in reads 14 kb or…
Our R&D team has been focusing on chemistry improvements for the PacBio® RS II sequencer and today we are pleased to announce our newest reagent combination, the P5 DNA polymerase with C3 chemistry (P5-C3). Together, they extend our industry-leading sequencing read lengths to an average of approximately 8.5 kb, with the longest reads exceeding 30,000 bases. The new chemistry includes photo-protected dyes to shield the P5 polymerase from laser-induced damage. With this new protection, the sequencing polymerase is able to generate much longer reads from a single DNA-template molecule. Our previously released chemistry, P4-C2, has been successfully used by many…
Pacific Biosciences is pleased to announce the introduction of DNA/Polymerase Binding Kit P4. This P4 enzyme has average read lengths of >4,300 bp when paired with the C2 sequencing chemistry and >5,000 bp when paired with the XL chemistry. The enzyme’s accuracy is similar to C2, reaching QV50 between 30X and 40X coverage. The resulting P4 attributes will provide you with higher-quality assemblies using fewer SMRT® Cells, and with improved variant calling. The P4 binding kit is compatible with PacBio® RS and PacBio RS II Systems with the latest version of Instrument Control Software (v1.3.3.1 and v2.0.1 respectively), and with…