Tuesday, April 16, 2019

Now Available: Low DNA Input Workflow for SMRT Sequencing

We are pleased to announce the availability of our protocol for template preparation to support low DNA input for sequencing on the Sequel System. The low DNA input workflow features a 3.5-hour library prep using the SMRTbell Express Template Prep Kit 2.0 (PN 100-938-900) from as little as 150 ng of input gDNA. This workflow can be used for de novo genome assembly of up to 300 Mb and can be scaled up for larger genomes with additional gDNA input. How low is low? This publication in the journal Genes shows how a team at the Wellcome Sanger Institute used…

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