Tuesday, August 1, 2017

To Resolve KIR Region, Scientists Pair SMRT Sequencing with Fosmid Cloning

A paper from scientists at the National Marrow Donor Program, Center for International Blood and Marrow Transplant Research, Fred Hutchinson Cancer Research Center, and other institutions reports the use of SMRT Sequencing to characterize the challenging killer cell immunoglobulin-like receptor (KIR) region in eight human genomes. By sequencing full-length fosmids, they found previously unreported haplotype structures. “Revealing Complete Complex KIR Haplotypes Phased By Long-Read Sequencing Technology” comes from lead author David Roe, senior author Martin Maiers, and collaborators. They targeted the KIR region — which has implications in autoimmune disease, transplantation, infections, and more — because it has historically been…

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