Wednesday, December 19, 2018

New Low-Input Protocol Enables High-Quality Genome Created from Single Mosquito

Anopheles coluzzii mosquito UPDATED January 18, 2019 This paper is now available at Genes. ORIGINAL POST December 19, 2018 High-quality reference and de novo genomes have been celebrated by geneticists, population biologists and conservationists alike, but it’s been a dream deferred for entomologists and others grappling with limited DNA samples, due to previous relatively high DNA input requirements (~5 μg for standard library protocol). A new low-input protocol now makes it possible to create high-quality de novo genome assemblies from just 100 ng of starting genomic DNA, without the need for time-consuming inbreeding or pooling strategies. The targeted release date for…

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