Wednesday, September 16, 2020

Easy and Affordable: Full-Length 16S HiFi Sequencing with PacBio Service Providers

Analysis of 16S ribosomal RNA has been used for phylogenetics and identifying prokaryotes for decades. But just as scientists have had to refine the Linnaean taxonomy system based on genomic discoveries, improvements in sequencing technology are changing 16S analysis best practices. Several dominant microbial genera in Sakinaw Lake could only be resolved via Full Length 16S or were missed by V4 sequencing (gray boxes). Singer, E. et al. (2016) Researchers at the Joint Genome Institute, for instance, conducted a detailed benchmarking study and found that traditional methods of 16S analysis — which look at just a piece of the gene…

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