Featured Documentation

Procedure & checklist — Extracting HMW DNA from cultured adherent cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured adherent cells.

Procedure & checklist — Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.

Procedure & checklist — Extracting HMW DNA from Gram-positive bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-positive bacteria.

Procedure & checklist — Extracting HMW DNA from human whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from human whole blood.

Procedure & checklist — Extracting HMW DNA from animal tissue using the Nanobind PanDNA kit

Description

This procedure can be used to extract HMW DNA from animal tissues using the Nanobind PanDNA kit

Procedure & checklist — Extracting HMW DNA from nucleated red blood cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from nucleated red blood cells using Nanobind kits.

SMRT Link User Guide v13.0

Description

This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.

Procedure & checklist — Extracting HMW DNA from human whole blood with RBC lysis using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from 400 μL of human whole blood with RBC lysis. It is recommended for PacBio® HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).

SMRT Link Release Notes v13.0

Description

Release notes for SMRT Link v13.0

Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio® Sequel® II and Revio™ systems

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Guide & overview — Nanobind PanDNA kit

Description

This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.

Procedure & checklist — Extracting DNA from cultured suspension cells using Nanobind kits

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from cultured suspension cells. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind CBB kit (102-301-900) or the Nanobind PanDNA kit (103-260-000).