Featured Documentation

Guide & overview — Nanobind PanDNA kit

Description

This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.

Overview — Nanobind CBB HMW DNA extraction protocols

Description

Overview – PacBio Nanobind CBB HMW DNA extraction protocols

Overview — Nanobind PanDNA HMW DNA extraction protocols

Description

Overview – PacBio Nanobind PanDNA HMW DNA extraction protocols

Procedure & checklist — Extracting HMW DNA from saliva collected in DNA Genotek Oragene devices using Nanobind kits

Description

This protocol describes the extraction of HMW DNA (50–300+ kb) from saliva collected with a DNA Genotek Oragene•DNA saliva collection device using Nanobind kits.

Guide & overview — Short Read Eliminator (SRE) XS and XL kits

Description

This document describes procedures on how to use the the SRE kit family (SRE XS, SRE XL) for rapid high-pass size selection of high molecular weight (HMW) DNA samples.

Overview — Nanobind HT HMW DNA extraction robotic procedures

Procedure & checklist — Extracting HMW DNA from human whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from human whole blood.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Apex system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Flex system

Procedure & checklist — Extracting HMW DNA from cultured adherent cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured adherent cells.

Procedure & checklist — Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.

Procedure & checklist — Extracting HMW DNA from Gram-positive bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-positive bacteria.

Procedure & checklist — Extracting HMW DNA from nucleated red blood cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from nucleated red blood cells using Nanobind kits.

Procedure & checklist — Extracting HMW DNA from human whole blood with RBC lysis using the Nanobind PanDNA kit

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from 400 μL of human whole blood with RBC lysis. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind PanDNA kit (103-260-000).