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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. See Other Documentation for product inserts, safety data sheets (SDS) and more.

May 8, 2024

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system
  • Automation, Library preparation

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.
May 8, 2024

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

  • Guide & overview
  • Revio system, Sequel II system and Sequel IIe system
  • Automation, Library preparation

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.
May 1, 2024

Guide & overview — Nanobind PanDNA kit

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Sample preparation

Description

This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
April 11, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 µL human whole blood on the KingFisher Flex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Automation

Description

This protocol describes the extraction of HMW (50–300 kb) DNA from 200 µL of human whole blood using the Thermo Fisher KingFisher Flex. It is recommended for HiFi sequencing. This protocol requires the Nanobind HT CBB kit (102- 762-700).
March 31, 2024

Guide & overview — Short Read Eliminator (SRE) XS and XL kits

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation

Description

This document describes procedures on how to use the the SRE kit family (SRE XS, SRE XL) for rapid high-pass size selection of high molecular weight (HMW) DNA samples.
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