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February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the KingFisher Apex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
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