April 21, 2020  |  

An efficient gene disruption system for the nematophagous fungus Purpureocillium lavendulum.

Authors: Liu, Lu and Cao, Yan-Ru and Zhang, Chen-Chen and Fan, Hai-Feng and Guo, Zhi-Yi and Yang, He-Yu and Chen, Mi and Han, Jing-Jing and Xu, Jianping and Zhang, Ke-Qin and Liang, Lian-Ming

The fungus Purpureocillium lavendulum (formally Paecilomyces lilacinus) is a natural enemy of insects and plant-parasitic nematodes, and has been used as an important bio-control agent against agricultural pests all over the world. In order to understand the genetic mechanisms governing its biocontrol efficiency and other biological processes, an effective gene disruption system is needed. Here we report the development of an efficient system which integrates selective markers that differ from Purpureocillium lilacinum, a one-step construction method for gene knockout plasmids, and a ku80 knockout strain for efficient homologous recombination. With this system, we effectively disrupted the transcription factors in the central regulation pathway of sporulation and a serine protease which were contributed to nematode infection, demonstrating this system as an efficient gene disrupting system for further characterization of genes involved in the development and pathogenesis of this fungus. Copyright © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Journal: Fungal biology
DOI: 10.1016/j.funbio.2018.10.009
Year: 2019

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