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Binding and Cleanup Kits for the Sequel System

DNA polymerase binding kits contain sequencing polymerase and reagents to prepare SMRTbell libraries for sequencing on the PacBio Systems. MagBead binding kits are used with polymerase-bound SMRTbell libraries for effective loading of DNA on SMRT Cells.

 

Sequel Binding and Internal Ctrl Kit 2.1

101-429-300
The Sequel Binding and Internal Cntrl Kit 2.1 is a bundle kit of Sequel Binding Kit 2.1 and Sequel DNA Internal Control Complex 2.1. The Sequel Binding Kit 2.1 contains reagent supplies to bind prepared DNA template libraries to the Sequel Polymerase 2.1 in preparation for sequencing on the Sequel System. The result is a DNA polymerase/template complex. Sequel Polymerase 2.1 should be used only with Sequel Sequencing Kit 2.1. Reagent quantities support 24 binding reactions. Sequel DNA Internal Control Complex 2.1 contains a fixed template of 2 kb length, bound to Sequel DNA Polymerase 2.1 for use as an internal sequencing control on the Sequel System. Reagent quantities provide spike-in controls for a minimum of 24 samples.

Sequel Binding and Internal Ctrl Kit 2.0

101-400-900
The Sequel Binding and Internal Cntrl Kit 2.0 is a bundle kit of Sequel Binding Kit 2.0 and Sequel DNA Internal Control Complex 2.0. The Sequel Binding Kit 2.0 contains reagent supplies to bind prepared DNA template libraries to the Sequel Polymerase 2.0 in preparation for sequencing on the Sequel System. The result is a DNA polymerase/template complex. Sequel Polymerase 2.0 should be used only with Sequel Sequencing Kit 2.0. Reagent quantities support 24 binding reactions. Sequel DNA Internal Control Complex 2.0 contains a fixed template of 2 kb length, bound to Sequel DNA Polymerase 2.0 for use as an internal sequencing control on the Sequel System. Reagent quantities provide spike-in controls for a minimum of 24 samples.

MagBead Kit v2

100-676-500
The MagBead Kit v2 contains reagent supplies to capture DNA polymerase and template complex for loading onto the PacBio Systems. Kit components include: MagBeads, MagBead Binding Buffer Kit v2 and Wash Buffer kit v2.
Procedure & Checklist: Preparing MagBeads for Sequencing

SMRTbell Clean Up Column v2 Kit-Dif

101-184-100
The SMRTbell Clean Up Column v2 Kit-Dif contains 20 columns and buffer to first condition the column with a compatible buffer for DIFFUSION loading of a DNA/polymerase complex. The SMRTbell Clean Up Column v2 removes excess free polymerase and primers from a DNA polymerase binding reaction. The SMRTbell Clean Up Column v2 Kit-Dif consists of 1 of each of the following parts: SMRTbell Clean Up Columns v2 (PN: 101-175-400) and MagBead Binding Buffer Kit v2 (PN: 101-046-400).

SMRTbell Clean Up Column v2 Kit-Mag

101-303-600
The SMRTbell Clean Up Column v2 Kit-Mag contains 20 columns and buffer to first condition the column with a compatible buffer for MAGBEAD loading of a DNA/polymerase complex. The SMRTbell Clean Up Column v2 removes excess free polymerase and primers from a DNA polymerase binding reaction. The SMRTbell Clean Up Column v2 Kit-Mag consists of 1 of each of the following parts: SMRTbell Clean Up Columns v2 (PN: 101-175-400) and MagBead Wash Buffer Kit v2 (PN: 101-046-500).

Loading Cleanup Binding Kit (10 rxn)

100-715-300
The Loading Cleanup Bead Kit (10 rxn) contains reagents to remove excess polymerase from a polymerase binding reaction. The Loading Cleanup Beads are only for diffusion loaded samples onto the Sequel SMRT Cell 1M. Reagent quantities support cleaning up 10 polymerase binding reactions.
Not applicable

MagBead

100-125-900
The MagBeads contains 2.88 mL of MagBeads to capture DNA polymerase and template complex for loading onto the PacBio systems. MagBeads are to be used with MagBead Binding and Wash Buffer Kits.
Not applicable

MagBead Wash Buffer Kit v2

101-046-500
The MagBead Wash Buffer Kit v2 is used with the MagBead Binding Buffer Kit and MagBeads using the Pacific Biosciences Binding Calculator to bind the DNA/Polymerase complex.
Not applicable

MagBead Binding Buffer Kit v2

101-046-400
The MagBead Binding Buffer Kit v2 is used with the MagBead Wash Buffer Kit and MagBeads using the Pacific Biosciences Binding Calculator to bind the DNA/Polymerase complex.
Not applicable

SMRTbell Clean Up Columns v2 (20 pack)

101-175-400

The SMRTbell Clean Up Columns v2 contain 20 columns, collection tubes and caps.

Not applicable

Not applicable

For more information about how our Sequel System consumables can support your research, contact us.

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