Sample and template preparation kits

Sample and template preparation kits for the PacBio RS II

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SMRTbell Template Prep Kit 1.0

100-259-100
The SMRTbell Template Prep Kit 1.0 contains reagent supplies to perform SMRTbell library preparations of non-size-selected or size-selected, primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to greater than 20 kb. Reagent quantities support 10 library preparations.

Insert: SMRTbell Template Prep Kit 1.0
SDS: SMRTbell Template Prep Kit 1.0

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 500bp Template Preparation and Sequencing

Procedure & Checklist: 20 kb Template Preparation Using BluePippin Size-Selection

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SMRTbell Barcoded Adapter Complete Prep Kit-96

100-514-900
The SMRTbell Barcoded Adapter Complete Prep Kit-96 contains both the SMRTbell Barcoded Adapter Prep Kit and Barcoded Adapter Plate-96. Reagent quantities support 96 individual barcoded adapter end-repair and ligation reactions. For use with the SMRTbell Damage Repair Kit to prepare primer-annealed SMRTbell libraries.

Insert: SMRTbell Barcoded Adapter Prep Kit
Insert: Barcoded Adapters Plate-96

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Barcoded Adapter Kit – 8A

101-081-300
The Barcoded Adapter Kit- 8A contains 8 blunt ended barcoded adapters and Sequencing Primer v4. Each tube of Barcoded Adapter contains sufficient reagents to perform 6 ligation reactions. The specific barcodes contained in this kit are: bc1001, bc1002, bc1004, bc1008, bc1009, bc1010, bc1012, bc1014. The barcodes are compatible with both PacBio RS II and Sequel Systems. Sequencing Primer v4 is designed for diffusion loading and is only tested on the Sequel System.

Insert: Barcoded Adapter Kit 8A
SDS: Barcoded Adapter Kit 8A

Procedure & Checklist: Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System

Analysis Procedure: Multiplexed Microbial Assembly with SMRT Link v5.1.0

Application Note: Microbial Multiplexing Workflow on the Sequel System

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Barcoded Adapter Kit – 8B

101-081-400
The Barcoded Adapter Kit – 8B contains 8 blunt ended barcoded adapters and Sequencing Primer v4. Each tube of Barcoded Adapter contains sufficient reagents to perform 6 ligation reactions. The specific barcodes contained in this kit are: bc1015, bc1016, bc1017, bc1018, bc1019, bc1020, bc1021, bc1022. The barcodes are compatible with both PacBio RS II and Sequel Systems. Sequencing Primer v4 is designed for diffusion loading and is only tested on the Sequel System.

Insert: Barcoded Adatper Kit 8B
SDS: Barcoded Adapter Kit 8B

Procedure & Checklist: Preparing Multiplexed Microbial SMRTbell Libraries for the PacBio Sequel System

Analysis Procedure: Multiplexed Microbial Assembly with SMRT Link v5.1.0

Application Note: Microbial Multiplexing Workflow on the Sequel System

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SMRTbell Damage Repair Kit

100-465-900
The SMRTbell Damage Repair Kit contains reagents to perform DNA damage repair and anneal sequencing primers. The SMRTbell Damage Repair Kit is used with the SMRTbell Barcoded Adapter Complete Prep Kit-96 to complete the SMRTbell library workflow for samples using barcoded adapters. Additionally, the SMRTbell Damage Repair kit can be used as a standalone DNA damage-repair reaction where applicable. Reagent quantities support 10 preparations.

Insert: SMRTbell Damage Repair Kit
SDS: SMRTbell Damage Repair Kit

Procedure & Checklist: Preparing SMRTbell Libraries using PacBio Barcoded Adapters for Multiplex SMRT Sequencing

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Barcoded Universal F/R Primer Plate-96

100-466-100
The Barcoded Universal F/R Primer Plate-96 contains both forward and reverse primers to be used in a PCR reaction to add barcodes to PCR amplicons that have been amplified with universal sequence tags. Each of 96 wells contains a different barcode and sufficient reagents for a single use. For use in conjunction with the SMRTbell Template Prep kit.

Insert: Barcoded Universal Primers Plate-96

Procedure & Checklist: Preparing SMRTbell Libraries using PacBio Barcoded Universal Primers for Multiplex SMRT Sequencing

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AMPure PB Beads

100-265-900
AMPure PB magnetic beads are specifically formulated for the SMRT Sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow.

Insert: Ampure PB
SDS: Ampure PB

The following is a sample of applicable protocols. For a complete list, please visit documentation and search for a protocol applicable to your research.

Procedure & Checklist: 500bp Template Preparation and Sequencing

Procedure & Checklist: 20 kb Template Preparation Using BluePippin Size-Selection

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